Abstract
Purpose : In recent years the infertile population applying for IVF treatments was changed and so the indications for performing intracellular sperm injection (ICSI). The aim of this study was to analyze predicting factors of our thawing cycles.
Methods : From December 1998 to July 2001, 440 consecutive thawing cycles were performed. Patient characteristics were examined. The number of cryopreserved embryos, number of transferred embryos, the timing of cryopreservation (48 h vs.72 h), and embryo survival rate were analyzed as a possible predictor for pregnancies achievement.
Results : Conventional IVF patient's characteristic was significantly different from ICSI population and analysis has been performed for every population separately. In the IVF population the women age, the number of transferred embryos, and timing of cryopreservation were factors significantly influencing the pregnancy rate. Interestingly, in the ICSI population only the number of transferred embryos was found to be a predictive factor.
Conclusion : ICSI and IVF cycles should be analyzed separately. Not all the factors influencing the success rate in the conventional IVF population are valid in the ICSI population.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Trounson A, Mohr L: Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 1983;305:707–709
Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainborn JD, Frydman R: High pregnancy rate after early human embryo freezing. Fertil Steril 1986;46:268–272
Camus M, Van Den Abbeel E, Van Waesberghe L, Wisanto A, Devroey P, Van Steirteghem AC: Human viability after freezing with dimethylsulfoxide as a cryoprotectant. Fertil Steril 1989;51:460–465
Van Der Elts J, Van Den Abbeel E, Vitrier S, Camus M, Devroey P, Van Steirteghem AC: Selective transfer of cryopreserved human embryos with further cleavage after thawing increases delivery and implantation rates. Hum Reprod 1997;12:1513–1521
Shapiro BS, Harris DC, Richter KS: Predictive value of 72-hour blastomere cell number on blastocyst development and success of subsequent transfer based on the degree of blastocyst development. Fertil Steril 2000;73:582–586
Dirnfeld M, Goldman S, Gonen Y, Koifman M, Lissak A, Kraiem Z, Abramovici H: Functional differentiation in progesterone secretion by granulosa versus cumulus cells in the human preovulatory follicle and the effect of different induction of ovulation protocols. Fertil Steril 1993;60:1025–1030
Dirnfeld M, Bider D, Koifman M, Calderon I, Abramovici H: Shortened exposure of oocytes to spermatozoa improves in-vitro fertilization outcome: A prospective, randomized, controlled, study. Hum Reprod 1999;14:2562–2564
Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde MP, Van Assche E, Devroey P: Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993;8:1055–1060
Lahav-Baratz S, Rothschild E, Grach B, Koifman M, Shiloh H, Ishai D, Dirnfeld M: The value of sperm pooling and cryopreservation in patients with transient azoospermia or severe oligoasthenoteratozoospermia. Hum Reprod 2002;17:157–160
Simon A, Holzer H, Hurwitz A, Revel A, Zentner B, Lossos F, Laufer N: Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization. J Assist Reprod Genet 1998;15:431–437
Kowalik A, Palremo GD, Barmat L, Veeck L, Rimarchin J, Rosenwaks Z: Comparison of clinical outcome after cryopreservation of embryos obtained from intracytoplasmic sperm injection and in-vitro fertilization. Hum Reprod 1998;13:2848–2851
Fugger EF: Clinical status of human embryo cryopreservation in the United States of America. Fertil Steril 1989;52:986–990
Fugger EF, Bustillo M, Dorfmann AD, Schulman JD: Human preimplantation embryo cryopreservation: Selected aspects. Hum Reprod 1991;6:131–135
Toner JP, Veeck LL, Acosta AA, Muasher SJ: Predictive value of pregnancy during original in vitro fertilization cycle on implantation and pregnancy in subsequent cryothaw cycles. Fertil Steril 1991;56:505–508
Lin YP, Cassidenti DL, Chacon RR, Soubra SS, Rosen GF, Yee B: Successful implantation of frozen sibling embryos is influenced by the outcome of the cycle from which they were derived. Fertil Steril 1995;63:262–267
Schalkoff ME, Oskowitz SP, Powers RD: A multifactorial analysis of the pregnancy outcome in a successful embryo cryopreservation program. Fertil Steril 1993;59:1070–1074
Janny L, Menezo YJ: Maternal age effect on early human embryonic development and blastocyst formation. Mol Reprod Dev 1996;45:31–37
Braude P, Bolton V, Moore S: Human gene expression first occurs between the four-and eight-cell stages of preimplantation development. Nature 1988;332:459–461
Kattera S, Shrivastav P, Craft I: Comparison of pregnancy outcome of pronuclear-and multicellular-stage frozen–thawed embryo transfers. J Assist Reprod Genet 1999;16:358–362
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lahav-Baratz, S., Koifman, M., Shiloh, H. et al. Analyzing Factors Affecting the Success Rate of Frozen–Thawed Embryos. J Assist Reprod Genet 20, 444–448 (2003). https://doi.org/10.1023/B:JARG.0000006705.46147.a2
Issue Date:
DOI: https://doi.org/10.1023/B:JARG.0000006705.46147.a2