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Spectrophotometric Methods for the Determination of Nelfinavir Mesylate

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Abstract

Three visual spectrophotometric methods are described for the assaying of nelfinavir mesylate (NEL) in either bulk form or dosage forms. Methods A-C are based on the oxidation of NEL with an excess of oxidant (N-bromosuccinimide (NBS) in methods A&B or chloramine-T (CAT) in method C) in acidic medium. The unreacted oxidant is then estimated colorimetrically using an oxidisable dye (Celistine blue (CB) in method A or Gallocyanine (GC) in method C) or p-N-methyl aminophenol sulphate (PMAP)-sulphanilamide (SA) reagent (in method B). These methods obey Beer's law and give reproducible results. Recoveries range from 99.3–100.5%.

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Authors and Affiliations

  1. Department of Organic Chemistry, Foods, Drugs and Water, School of Chemistry, Andhra University, Visakhapatnam, 530 003, India

    Seshagiri V. M. Mohan Rao & Chilukuri S. P. Sastry

  2. Department of Physical and Nuclear Chemistry & Chemical Oceanography, School of Chemistry, Andhra University, Visakhapatnam, 530 003, India

    Tadi R. S. Reddy & Isukapatla N. Rao

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  1. Seshagiri V. M. Mohan Rao
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  2. Tadi R. S. Reddy
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  3. Isukapatla N. Rao
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  4. Chilukuri S. P. Sastry
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Correspondence to Chilukuri S. P. Sastry.

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Mohan Rao, S.V.M., Reddy, T.R.S., Rao, I.N. et al. Spectrophotometric Methods for the Determination of Nelfinavir Mesylate. Journal of Analytical Chemistry 59, 552–556 (2004). https://doi.org/10.1023/B:JANC.0000030876.80807.dc

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  • DOI: https://doi.org/10.1023/B:JANC.0000030876.80807.dc

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