Abstract
For increasing the contents of specificfree amino acids in rice (Oryzasativa L.) cultivar, Donganbyeo, mutantcell lines resistant to growth inhibitionby 5-methyltryptophan (5MT) were selectedfrom embryo cultured callus irradiated with50 Gy of gamma rays. Two lines, M2-1and M2-2, were obtained from theregenerated plants by selfing. Thesegregation ratios of resistance andsensitivity in these lines fitted 9:7 and3:1 ratios, respectively. Considering theagronomic traits, M2-1 was about thesame as the original variety in culm lengthand a little longer or higher in paniclelength and number of tillers. However, thefertility of M2-1 significantlyincreased compared to the originalvariety. M2-2 had an extremely highculm length and tillering capacity. Four5MT-resistant homozygous M3lines (MRclasses), M3-1-40 and M3-1-116from M2-1 (MR I),and M3-2-8 and M3-2-12from M2-2 (MR II), were obtained inthe same manner as with the M2generation. Protein contents of brown ricewere increased about 19% and 32% and the total contents of 9 free essentialamino acids were 71% and 34% greater thanthe original variety in the MR I andMR II groups, respectively. However, forthe free essential amino acids of polishedrice, there was no difference from theoriginal variety.
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References
Brotherton, J.E., R.M. Hauptmann & J.M. Widholm, 1986. Anthranilate synthase forms in plants and cultured cells of Nicotiana tabacum L. Planta 168: 214–221.
Carlson, P.S., 1970. Induction and isolation of auxotrophic mutants in somatic cell cultures of Nicotiana tabacum. Science 168: 487–489.
Carlson, P.S., 1973. Methionine sulfoximine-resistant mutants of tobacco. Science 180: 1136–1138.
Chowdari, K.V., W. Ramakrishna, S.A. Tamhankar, R.R. Hendre, V.S. Gupta, N.A. Sahasrabudhe & P.K. Ranjekar, 1998. Identification of minor DNA variation in rice somaclonal variants. Plant Cell Rep 18: 55–58.
Chu, C.C., C.C. Wang, C.S. Sun, C. Hsu, K.C. Yin, C.Y. Chu & F.I. Bi, 1975.Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18: 659–668.
Green, C.E. & R.L. Phillips, 1974. Potential selection system for mutants with increased lysine, threonine, and methionine in cereal crops. Crop Sci 14: 827–830.
Jacobsen, E., R.G.F. Visser & J. Wijbrandi, 1985. Penylalanine and tyrosine accumulating cell lines of dihaploid potato selected by resistance to 5-methyltryptophan. Plant Cell Rep 4: 151–154.
Kang, K.K. & T. Kameya, 1995. Characterization of anthranilate synthetase and tryptophan synthase in a 5-methyltryptophan resistant mutant (MR1) of Zea mays L. Breeding Sci 45: 321–325.
Kisaka, H., M. Kisaka & T. Kameya, 1996. Characterization of interfamilial somatic hybrids 5-methyltryptophan resistant rice (Oryza sativa L.) and 5MT-sensitive carrot (Daucus carota L.); expression of resistance to 5MT by the somatic hybrids. Breeding Sci 46: 221–226.
Larkin, P.J. & W.R. Scowcroft, 1981. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214.
Lee, H.Y. & T. Kameya, 1991. Selection and characterization of a rice mutant resistant to 5-methyltryptophan. Theor Appl Genet 82: 405–408.
Lee, H.Y., J.H. Kim, H.J. Kim, & T. Kameya, 1992. Selection and rice cell lines resistant to 5-methyltryptophan in cell cultures. Korean J Plant Tissue Cult 19: 289–294.
Lee, Y.I., 1977. Induction and selecting of useful mutants in cultured plant cells. Korea J Plant Tissue Cult 5: 37–42.
Lee, Y.I., K.K. Kang & S.J. Lee, 1996. Somaclonal variation induced by in vitro mutagenesis in sweet potato. In: Plant Biotechnology for Sustainable Development of Agriculture, pp. 90-96. Proceeding of 2nd Asia-Pacific Conference on Plant Cell and Tissue Culture, July 28-August 1, 1996. Beijing, China.
Mori, S., H. Haregawa, R. Che, H. Nakanishi & M. Murakami, 1989. Free proline contents in two different groups of rice mutants resistant to Hydroxy-L-proline. Theor Appl Genet 77: 44–48.
Nabors, M.W., 1976. Using spontaneously occurring and induced mutations to obtain agriculturally useful plant. BioScience 26: 761–768.
Nelson, O.E., 1969. Genetic modification of protein quality in plants. Advan Agron 21: 171–194.
Nelson, O.E., 1977. The applicability of plant cell and tissue culture techniques to plant improvement. In: I. Rubenstein, R. Phillips & C.E. Green (Eds.), Molecular Genetic Modification of Eukaryotes, pp. 67–76. Academic Press, New York.
Oono, K., 1981. In vitro methods applied to rice. In: T.A. Thorpe (Ed.), Plant Tissue Culture Methods and Application in Agriculture, pp. 273–298. Academic press, New York.
Phillips, R.L., S.M. Kaeppler & P. Olhoft, 1994. Genetic instability of plant tissue cultures: Breakdown of normal controls. Proc Natl Sci USA. 91: 5222–5226.
Ranch, J.P., S. Rick, J.E. Brotherton & J.M. Widholm, 1983. Expression of 5-methyltryptophan resistance in plants regenerated from resistant cell lines of Datura innoxia. Plant Physiol 71: 136–140.
Schaeffer, G.W., 1981. Mutations and cell selections: increased protein from regenerated rice tissue cultures. Env Exp Bot 21: 333–345.
Schaeffer, G.W. & F.T. Sharpe, 1983. Mutations and Selection: Genetic Variation for Improved Protein in Rice. Genetic Engineering, Application to Agriculture; Invited papers presented at a symposium, May 16-19, 1982, Beltsville, pp. 237-254.
Steinke, F.H. & D.T. Hopkins, 1983. Complementary and supplementary effects of vegetable proteins. Cereal Foods World 28: 338–341.
Tarr, G.E., 1986. Methods of protein microcharacterization. In: J.E. Shively (Ed.), Protein Methods, pp. 155–194. Humana press, Clifton, NJ.
Wakasa, K., 1985. Mutation selection in vitro in rice and tobacco. In: G.M. Reddy & E.H. Jr. Coe (Eds.), Gene Structure and Function in Higher Plants, pp. 247–251. Oxford & IBH Pulb, New Delhi.
Wakasa, K. & J.M.Widholm, 1987. A 5-methyltryptophan resistant rice mutant, MTR1, selected in tissue culture. Theor Appl Genet 74: 49–54.
Widholm, J.M., 1971. Control of tryptophan biosynthesis in plant tissue cultures: lack of repression of anthranilate and tryptophan synthetases by tryptophan. Physiol Plant 25: 75–79.
Widholm, J.M., 1972. Anthranilate synthetase from 5-methyltryptophan-susceptible and resistant cultured Daucus carota cells. Biochim Biophys Acta 279: 48–57.
Widholm, J.M., 1973. Measurement of the five enzymes which convert chorismate to tryptophan in cultured Daucus carota cell extracts. Biochim Biophys Acta 320: 217–226.
Widholm, J.M., 1974. Cultured carrot cell mutants: 5-methyltryptophan-resistant trait carried from cell to plant and back. Plant Sci Lett 3: 323–330.
Widholm, J.M., 1977. Selection and characterization of amino acid analog resistant plant cell culture. Crop Sci 17: 597–600.
Yang, H., Y. Tabei, H. Kamada & T. Kayano, 1999. Detection of somaclonal variation in cultured rice cells using digoxigeninbased random amplified polymorphic DNA. Plant Cell Rep 18: 520–526.
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Kim, D., Lee, I., Jang, C. et al. Selection of 5-methyltryptophan resistant rice mutants from irradiated calli derived from embryos. Euphytica 135, 9–19 (2004). https://doi.org/10.1023/B:EUPH.0000009509.78515.8e
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DOI: https://doi.org/10.1023/B:EUPH.0000009509.78515.8e