CHO-K1 and VERO cells have been grown on MicroHex™, a polystyrene-based microsupport with two-dimensional geometry and the consistency of their growth kinetics were established. These cells have been detached by exposure to buffered ethylenediaminetetraacetic acid (EDTA) followed by controlled mechanical shear to yield well-isolated cells suspended in EDTA. Neutralisation of the EDTA followed by restoration of the chemical composition of the growth medium causes detached CHO-K1 cells to display unaltered growth kinetics.
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- Bio Directory (2002) Amersham Biosciences, pp. 53–54.Google Scholar
- Chowdhury JR, Chowdhury NR, Demetrious AA & Wilson JM (1989) Use of microbeads for cell transplantation. In: Miller AOA (ed.) Advanced Research in Animal Cell Technology (pp. 315–317) Kluwer Academic Publishers, Dordrecht.Google Scholar
- Jones G (1991) Measurement of Cell Adhesion in Suspension. In: Curtis ASG & Lackie JM (eds) Measuring Cell Adhesion (pp. 23–39) John Wiley & Sons, New york.Google Scholar
- Kenda-Ropson N, Mention D, Motte V, Genlain M & Miller AOA (2002a) Microsupport with two-dimensional geometry (2D-MS). 3. In situ determination of the growth kinetics of anchorage-dependent cells by laser diffraction particle sizing (LDPS). Cytotechnology 37: 49–53.Google Scholar
- Kenda-Ropson N, Lenglois S & Miller AOA (2002b) Microsupport with two-dimensional geometry (2D-MS). 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHexTM (CryoHex). Cytotechnology 39: 163–170.Google Scholar
- Merten O-W, Dante J, Noguiez-Hellin P, Laune S, Klatzmann D, Salzmann J-L (1997) New process for cell detachment: use of heparin. In: Corrondo MJT, Griffiths JB Moreira JLP (eds) Animal cell Technology: From Vaccines to Genetic Medecine (pp. 343–348) Kluwer Academic Press, Dordrecht.Google Scholar
- Merten O-W (1999) Cell detachment. In: Spier RE (ed.) The Encyclopedia of Cell Technology (pp. 351–365) John Wiley & Sons, New York.Google Scholar
- Miller AOA, Menozzi FD & Dubois D (1989) Microbeads and anchorage-dependent eukaryotic cells: the beginning of a new era in biotechnology. In: Fiechter A (ed.) Advances in Biochemical Engineering/Biotechnology, Vol. 39 (pp. 73–95) Springer Verlag, Berlin.Google Scholar
- Van Wezel AL (1967) Growth of cell-strains and primary cells on microcarriers in homogeneous culture. Nature 216: 64–65.Google Scholar