An interspecific cross was made to transfer leaf rust and stripe rust resistance from an accession of Aegilops ovata (UUMM) to susceptible Triticum aestivum (AABBDD) cv. WL711. The F1was backcrossed to the recurrent wheat parent, and after two to three backcrosses and selfing, rust resistant progenies were selected. The C-banding study in a uniformly leaf rust and stripe rust resistant derivative showed a substitution of the 5M chromosome of Ae. ovata for 5D of wheat. Analysis of rust resistant derivatives with mapped wheat microsatellite makers confirmed the substitution of 5M for 5D. Some of these derivatives also possessed one or more of the three alien translocations involving 1BL, 2AL and 5BS wheat chromosomes which could not be detected through C-banding. A translocation involving 5DSof wheat and the substituted chromosome 5M of Ae. ovata was also observed in one of the derivatives. Susceptibility of this derivative to leaf rust showed that the leaf rust resistance gene(s) is/are located on short arm of 5M chromosome of Ae. ovata. Though the Ae. ovatasegment translocated to 1BL and 2AL did not seem to possess any rust resistance gene, the alien segment translocated to 5BS may also possess gene(s) for rust resistance. The study demonstrated the usefulness of microsatellite markers in characterisation of interspecific derivatives.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Bai, D.P., G.J. Scoles & D.R. Knott, 1994. Transfer of leaf rust and stem rust resistance genes from Triticum triaristatum to durum and bread wheat and their molecular cytogenetic localization. Genome 37(3): 410–418.
Dhaliwal, H.S. & Harjit-Singh, 1997. Breeding for resistance to bunts and smuts: Indian scenario. In: Bunts and Smuts of Wheat: An International Symposium, pp. 327–347. North American Plant Protection Organization, North Carolina, USA.
Dhaliwal, H.S, Harjit-Singh, K.S. Gill & H.S. Randhawa, 1993. Evaluation and cataloguing of wheat genetic resources for disease resistance and quality, In: A.B. Damania (Ed.), Biodiversity and Wheat Improvement, pp. 123–140. John Wiley and Sons, Chichester, U.K.
Dhaliwal, H.S, Harjit-Singh, S. Gupta, P.S. Bagga & K.S. Gill, 1991. Evaluation of Aegilops and wild Triticum species for resistance to leaf rust (Puccinia recondita f.sp. tritici) of wheat. Intern J Trop Agric 9(2): 118–121.
Friebe, B., F.J. Zeller, Y. Mukai, B.P. Foster, P. Bartos & R.A. McIntosh, 1992. Characteristics of rust resistant wheat-Agropyron intermedium derivatives by C-banding, in situ hybridization and isozyme analysis. Theor Appl Genet 83: 775–785.
Friebe, B., J. Jiang, W.J. Raupp, R.A. McIntosh & B.S. Gill, 1996. Characterization of wheat alien translocation conferring resistance to diseases and pest: Current status. Euphytica 91: 59–87.
Gale, M.D. & T.E. Miller, 1987. The introduction of alien genetic variation into wheat. In: F.G.H. Lupton (Ed.), Wheat Breeding - Its Scientific Basis, pp. 173–210. Chapman and Hall, U.K.
Gill, B.S., B. Fribe & T.R. Endo, 1991. Standard karyotype and nomenclature system for description of chromosome bands and structural aberration in wheat (Triticum aestivum). Genome 34: 830–839.
Harjit-Singh & H.S. Dhaliwal, 1996. Transfer of rust and cereal cyst nematode resistance from Aegilops ovata to Triticum aestivum L. In: Sustainable Agriculture-Second Int Crop Sci Congr, New Delhi, p. 454 (v).
Harjit-Singh, H.S. Dhaliwal, J. Kaur & K.S Gill, 1993. Rust resistance and chromosome pairing in Triticum × Aegilops crosses. Wheat Inf Serv 76: 23–26.
Harjit-Singh, T.S. Grewal, H.S. Dhaliwal, P.P.S. Pannu & P.S. Bagga, 1998. Sources of leaf rust and stripe rust resistance in wild relatives of wheat. Crop Improv 256(1): 26–33.
Jauhar, P.P., 1993. Alien gene transfer and genetic enrichment of bread wheat. In: A.B. Damania (Ed.), Biodiversity and Wheat Improvement, pp. 103–119. John Wiley and Sons. Chichester, UK.
Jiang, J., B. Friebe & B.S. Gill, 1994. Recent advances in alien gene transfer in wheat. Euphytica 73: 199–212.
Jiang, J., B. Friebe, H.S. Dhaliwal, T.J. Martin & B.S. Gill, 1993. Molecular cytogenetic analysis of Agropyron elongatum chromatin in wheat germplasm specifying resistance to wheat streak mosaic virus. Theor Appl Genet 86: 41–48.
McIntosh, R.A., 1998. Catalogue of gene symbols for wheat. In: A.E. Slinkard (Ed.), Proc 9th Int Wheat Genet Symp Vol. 5. University Extension Press, Univ of Saskatchewan, Saskatoon Saskatchewan, Canada.
Mukai, Y. & B.S. Gill, 1991. Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome 34: 448–452.
Nayar, S.K., M. Prashar & S.C. Bhardwaj, 1997. Manual of Current Techniques in Wheat Rust. Research Bulletin No. 2. Directorate of Wheat Research, Flowerdale, Shimla. 32 pp.
Peterson, R.F., A.B. Campbell & A.E. Hannali, 1948. A diagramatic scale for estimating rust intensity on leaves and stems of cereals. Canadian J Res Section C-26: 496–563.
Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M. Tixier, P. Leroy & M.W. Ganal, 1998. A microsatellite map of wheat. Genetics 149: 2007–2023.
Schachermayr, G.M., M.M. Messmer, C. Feuillet, H. Winzeler, M. Winzler & B. Keller, 1995. Identification of molecular markers linked to the Agropyron elongatom - derived leaf rust resistance gene Lr24 in wheat. Theor Appl Genet 90: 982–990.
Sharma, H.C. & B.S. Gill, 1983. Current status of wide hybridization in wheat. Euphytica 32: 17–31.
Stakman, E.C., D.M. Stewart & W.Q. Loegering, 1962. Identification of physiological races of Puccinia recondita var. tritici. Miss Agri Expt Sta Sci J Series paper 4691.
About this article
Cite this article
Dhaliwal, H., Harjit-Singh & William, M. Transfer of rust resistance from Aegilops ovata into bread wheat (Triticum aestivum L.) and molecular characterisation of resistant derivatives. Euphytica 126, 153–159 (2002). https://doi.org/10.1023/A:1016312723040
- Microsatellite markers
- Puccinia recondita f.sp. tritici
- Puccinia striiformis
- rust resistance