Skip to main content
SpringerLink
Log in

Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Diversity in 28 accessions representing 12 species of the genus, Cajanus arranged in 6 sections including 5 accessions of the cultivated species, C. cajan, and 4 species of the genus Rhyncosia available in the germplasm collection at ICRISAT was assessed using RFLP with maize mtDNA probes. Cluster analysis of the Southern blot hybridization data with 3 restriction enzymes – 3 probe combinations placed the genus Rhyncosia in a major group well separated from all the species belonging to the genus Cajanus. Within the genus Cajanus, the 4 accessions of C. platycarpus belonging to section Rhynchosoides formed a separate group in contrast to those in other sections of pigeonpea. In the section, Cajanus all the 5 accessions of C. cajan were grouped together and C. cajanifolius belonging to the same section was in a subgroup by itself closer to the main group. The four accessions of C. scarabaeoides, were together and the other species belonging to section Cantharospermum were in different subgroups. The intra-specific variation was seen even within accessions of certain pigeonpea wild species such as C. scarabaeoides, C. platycarpus, C. acutifolius, and even the cultivated species of C. cajan. This study suggests that RFLP of mtDNA can be used for the diversity analysis of pigeonpea and it gives some indications on the maternal lineage among the species. The variations in the mitochondrial DNA hybridization patterns also suggest the extensive rearrangement of the organelle genome among the Cajanus species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmad, F., P.M. Gur & A.E. Slinkard, 1992. Isozyme polymorphism and phylogenetic interpretations in the genus Cicer L. Theor Appl Genet 83: 620–627.

    Article  Google Scholar 

  • Close, P.S., R.C. Shoemaker & P. Keim, 1989. Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine subgenus Soja. Theor Appl Genet 77: 768–776.

    Article  CAS  Google Scholar 

  • Deu, M.P., Hamon, J. Chantereau, P. Dufour, A. D'hont & C. Lanaud, 1995. Mitochondrial DNA diversity in wild and cultivated sorghum. Genome 38: 635–645.

    PubMed  CAS  Google Scholar 

  • Devos, K.M. & M.D. Gale, 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84: 567–572.

    Article  Google Scholar 

  • Dewey, R.E., C.S. Levings III & D.H. Timothy, 1985. Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79: 914–919.

    Article  PubMed  CAS  Google Scholar 

  • Ennes R.A., W.T. Sinclair, X-S. Hu & A. Langdon, 1999. Using organelle markers to elucidate the history, ecology and evolution of plant populations. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 1–19. Taylor & Francis, London.

    Google Scholar 

  • Feinburg, A.P. & B. Vogelstein, 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267.

    Google Scholar 

  • Gawel, N.J., R.L. Jarret & A. Whittemore, 1992. Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa. Theor Appl Genet 84: 286–290.

    Article  Google Scholar 

  • Gonzalez, J.M. & E. Ferrer, 1993. Random amplified polymorphic DNA analysis in Hordeum species. Genome 36: 1029–1031.

    CAS  PubMed  Google Scholar 

  • Grabau, E.A., W.H. Davis, N.D. Phelps & B.G. Gengenbach, 1992. Classification of soybean cultivars based on mitochondrial restriction fragment length polymorphisms. Crop Sci 32: 271–274.

    Article  CAS  Google Scholar 

  • Harris, S.A., 1999. RAPDs in sytematics - a useful methodology? In: P.M. Hollingsworth, R.M. Bateman and R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 211–228. Taylor & Francis, London.

    Google Scholar 

  • Hershkovitz, M.A., E.A. Zimmer & W.J. Hahn, 1999, Ribosomal DNA sequences and angiosperm systematics. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 268–326. Taylor & Francis, London.

    Google Scholar 

  • Hongtrakul, V., G.M. Huestis & S.J. Knap, 1997. Amplified fragment length polymorphisms as tool for DNA fingerprinting sunflower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95: 400–407.

    Article  CAS  Google Scholar 

  • Isaac, P.G., V.P. Jones & C.J. Leaver, 1985. The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male-sterile plants. EMBO J 4: 1617–1623.

    PubMed  CAS  Google Scholar 

  • Jain, A., S. Bhatia, S.S. Banga, S. Prakash & M. Lakshmikumaran, 1994. Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet: 116–122.

    Google Scholar 

  • Jarret, R.L., N. Gawel & A. Whittemore, 1992. Phylogenetic relationships of the sweet potato (Ipomoea batatas (L.) Lam.] J Am Soc Hort Sci 117: 633–637.

    Google Scholar 

  • Jena, K.K. & G.C. Kochert, 1991. Restriction fragment length polymorphism analysis of CCDD genome species of the genus Oryza L. Plant Mol Biol 16: 831–839.

    Article  PubMed  CAS  Google Scholar 

  • Krishna, T.G. & L.J. Reddy, 1982. Species affinities between Cajanus cajan and some Atylosia species based on esterase isozymes. Euphytica 31: 709–713.

    Article  Google Scholar 

  • Ladizinsky, G. & A. Hamel, 1980. Seed protein profiles of pigeonpea (Cajanus cajan) and some Atylosia species. Euphytica 29: 313–317.

    Article  CAS  Google Scholar 

  • Levinson, G. & G.A. Gutman, 1987. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Molec Biol Evol 4: 203–221.

    PubMed  CAS  Google Scholar 

  • Mackill, D.J., 1995. Classifying Japonica rice cultivars with RAPD markers. Crop Sci 35: 889–894.

    Article  CAS  Google Scholar 

  • McGregor C.E., C.A. Lambert, M.M. Greyling, J.H. Louw & L. Warnich, 2000. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135–144.

    Article  CAS  Google Scholar 

  • Miller, J.C. & S.D. Tanksley, 1990. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80: 437–448.

    CAS  Google Scholar 

  • Moeykens, C.A., S.A. Mackenzie & R.C. Shoemaker, 1995. Mitochondrial genome diversity in soybean: repeats and rearrangements. Plant Mol Biol 29: 245–254.

    Article  PubMed  CAS  Google Scholar 

  • Nadimpalli, R.G., R.L. Jarret, S.C. Phatak & G. Kochert, 1993. Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms. Genome 36: 216–223.

    PubMed  CAS  Google Scholar 

  • Nei, M & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci (USA) 76: 5269–5273.

    Article  CAS  Google Scholar 

  • Nene, Y.L. & V.K. Sheila, 1990. Pigeonpea: Geography and importance. In: Y.L. Nene, S.D. Hall & V.K. Sheila (Eds.), The Pigeonpea, pp. 1-15. C.A.B. International.

  • Provan, J., N. Saranzo, N.J. Wison, J.W. McNicol, M. Margante & W. Powel, 1999. The use of uniparentally inherited simple sequence repeat markers in plant population studies and systematics. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 35–50. Taylor and Francis, London.

    Google Scholar 

  • Pundir, R.P.S. & R.B. Singh, 1985. Cytogenetics of F1 hybrids between Cajanus and Atylosia species and its phylogenetic implications. Theor Appl Genet 71: 216–220.

    Google Scholar 

  • Ratnaparkhe, M.B., V.S. Gupta, M.R. Ven Murthy & P.K. Ranjekar, 1995. Genetic fingerprinting of pigeonpea (Cajanus cajan (L.) Millsp.] and its wild relatives using RAPD markers. Theor Appl Genet 91: 893–898.

    Article  CAS  Google Scholar 

  • Reddy, L.J. & D.N. De, 1983. Cyto-morphological studies in Cajanus cajan ?Atylosia lineata. Ind J Genet 43: 96–103.

    Google Scholar 

  • Reddy, L.J., J.M. Green & D. Sharma, 1981. Genetics of Cajanus cajan (L.) Mills sp. ?Atylosia spp. In: Proceedings of the International workshop on Pigeonpeas, 15-19 Dec 1980. ICRISAT Center, Vol 2, pp. 39–50. Patancheru, AP, India.

    Google Scholar 

  • Singh, A.K., S. Sivaramakrishnan, M.H. Mengesha & C.D. Ramaiah, 1991. Phylogenetic relations in section Arachis based on seed protein profile. Theor Appl Genet 82: 593–597.

    Google Scholar 

  • Sivaramakrishnan, S., K. Seetha, A. Nageshwar Rao & Laxman Singh, 1997. RFLP analysis of Cytoplasmic male-sterile lines of pigeonpea [Cajanus cajan (L.) Millsp.] developed by interspecific crosses. Euphytica 93: 307–312.

    Article  CAS  Google Scholar 

  • Smartt, J., 1990. Grain Legumes: Evolution and Genetic Resources, pp. 278–293. Cambridge University Press, Cambridge.

    Google Scholar 

  • Svitashev, S., T. Bryngelsson, A. Vershinin, C. Pedersen, T. Sall & R. von Bothmer, 1994. Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. Theor Appl Genet 89: 801–810.

    Article  CAS  Google Scholar 

  • Tozuka, A., H. Fukushi, T. Hirata, M. Ohara, A. Kanazawa, T. Mikami, J. Abe & Y. Shimamoto, 1998. Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA. Theor Appl Genet 96: 170–176.

    Article  CAS  Google Scholar 

  • van der Maesen, L.J.G., 1986. Cajanus D.C. and Atylosia W. & A. (Leguminosae). Agric. University of Wageningen Papers 85-4 (1985). Agricultural University. Wageningen, The Netherlands, 225 pp.

    Google Scholar 

  • van der Maesen, L.J.G., 1990. Pigeonpea: origin, history, evolution, and taxonomy. In: Y.L. Nene, S.D. Hall & V.K. Sheila (Eds.), The Pigeonpea, pp. 15-46. C.A.B. International.

  • Wang, G., R. Mahalingam & H.T. Knap, 1998. (C-A) and (GA) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr. Theor Appl Genet 96: 1086–1096.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ICRISAT, International Crops Research Institute for the Semi-Arid Tropics (, Patancheru, A.P, 502 324, India

    S. Sivaramakrishnan, Seetha Kannan & L.J. Reddy

Authors
  1. S. Sivaramakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seetha Kannan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L.J. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sivaramakrishnan, S., Kannan, S. & Reddy, L. Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA. Euphytica 125, 21–28 (2002). https://doi.org/10.1023/A:1015759318497

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015759318497

Search

Navigation