Abstract
1. We used the quantitative receptor autoradiographic method plus 125I-endothelin-1 (125I-ET-1), BQ-123, a specific antagonist for the endothelin ETA receptor, and sarafotoxin S6c, a selective agonist for the ETB receptor to investigate the ET receptor in the rat pituitary gland.
2. The method revealed that the BQ-123-sensitive ETA receptor was present predominantly in the anterior lobe and Rathke's pouch.
3. The posterior lobe contained BQ-123-sensitive ETA and sarafotoxin S6c-sensitive ETB receptors, in almost the same proportion. There was no significant 125I-ET-1 binding to the intermediate lobe.
4. Knowledge of the heterogeneous distribution of ET receptor subtypes in the pituitary gland supplies information that will be pertinent to physiological investigations of the gland.
Similar content being viewed by others
REFERENCES
Arai, H., Hori, S. Aramori, I., Ohkubo, H., and Nakanishi, S. (1990). Cloning and expression of a cDNA encoding an endothelin receptor. Nature 348:730–732.
Carmel, P. W. (1985). Craniopharyngiomas. In Wilkins, R. H., and Rengachary, S. S. (eds.), Neurosurgery, McGraw-Hill, New York, pp. 905–916.
Dickinson, K. E. J., Tymiak, A. A., Cohen, R. B., Liu, E. C.-K., Webb, M. L., and Hedberg, A. (1991). Vascular A10 cell membranes contain an endothelin metabolizing neutral endopeptidase. Biochem. Biophys. Res. Commun. 176:423–430.
Domae, M., Yamada, K., Hanabusa, Y., and Furukawa, T. (1992). Inhibitory effects of endothelin-1 and endothelin-3 on prolactin release: possible involvement of endogenous endothelin isopeptides in the rat anterior pituitary. Life Sci. 50:715–722.
Economos, K., MacDonald, P. C., and Casey, M. L. (1992). Endothelin-1 gene expression and biosynthesis in human endometrial HEC-1A cancer cells. Cancer Res. 52:554–557.
Gross, P. M., Wainman, D. S., and Espinosa, F. J. (1991). Differentiated metabolic stimulation of rat pituitary lobes by peripheral and central endothelin-1. Endocrinology 129:1110–1112.
Hama, H., Sakurai, T., Kasuya, Y., Fujiki, M., Masaki, T., and Goto, K. (1992). Action of endothelin-1 on rat astrocytes through the ETB receptor. Biochem. Biophys. Res. Commun. 186:355–362.
Hirata, Y., Emori, T., Eguchi, S., Kanno, K., Imaki, T., Ohta, K., and Marumo, F. (1993). Endothelin receptor subtype B mediates synthesis of nitric oxide by cultured bovine endothelial cells. J. Clin. Invest. 91:1367–1373.
Ihara, M., Noguchi, K., Saeki, T., Fukuroda, T., Tsuchida, S., Kimura, S., Fukami, T., Ishikawa, K., Nishikibe, M., and Yano, M. (1992). Biological profiles of highly potent novel endothelin antagonists selective for the ETA receptor. Life Sci. 50:247–255.
Israel, A., Correa, F. M. A., Niwa, M., and Saavedra, J. M. (1984). Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography. Brain Res. 322:341–345.
Kanyicska, B., and Freeman, M. E. (1993). Characterization of endothelin receptors in the anterior pituitary gland. Am. J. Physiol. 265:E601–E608.
Kitagawa, N., Tsutsumi, K., Niwa, M., Himeno, A., Yamashita, K., Shibata, S., Taniyama, K., Kurihara, M., Kawano, T., Yasunaga, A., and Shibata, S. (1994). Expression of a functional endothelin (ETA) receptor in human meningiomas. J. Neurosurg. 80:723–731.
MacCumber, M. W., Ross, C. A., and Snyder, S. H. (1990). Endothelin in brain: Receptors, mitogenesis, and biosynthesis in glial cells. Proc. Natl. Acad. Sci. USA 87:2359–2363.
McPherson, G. A. (1985). Analysis of radioligand binding experiments. A collection of computer programs for the IBMPC. J. Pharmacol. Methods 14:213–228.
Munson, P. J., and Rodbard, D. (1980). LIGAND: a versatile computerized approach for characterization of ligand-binding systems. Anal. Biochem. 107:220–239.
Nakamura, S., Naruse, M., Naruse, K., Shioda, S., Nakai, Y., and Uemura, H. (1993). Colocalization of immunoreactive endothelin-1 and neurohypophysial hormones in the axons of the neural lobe of the rat pituitary. Endocrinology 132:530–533.
Nambi, P., Wu, H. L., Pullen, M., Aiyar, N., Bryan, H., and Elliott, J. (1992). Identification of endothelin receptor subtypes in rat kidney cortex using subtype-selective ligands. Mol. Pharmacol. 42:336–339.
Naruse, M., Naruse, K., Nishikawa, T., Yoshihara, I., Ohsumi, K., Suzuki, N., Demura, R., and Demura, H. (1992). Endothelin-3 immunoreactivity in gonadotrophs of the human anterior pituitary. J. Clin. Endocrinol. Metab. 74:968–972.
Niwa, M., Kawaguchi, T., Himeno, A., Fujimoto, M., Kurihara, M., Yamashita, K., Kataoka, Y., Shigematsu, K., and Taniyama, K. (1992). Specific binding sites for 125I-endothelin-1 in the porcine and human spinal cord. Eur. J. Pharmacol. Mol. Pharmacol. 225:281–289.
Sakurai, T., Yanagisawa, M., Takuwa, Y., Miyazaki, H., Kimura, S., Goto, K., and Masaki, T. (1990). Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor. Nature 348:732–735.
Samson, W. K. (1992). The endothelin-A receptor subtype transduces the effects of the endothelins in the anterior pituitary gland. Biochem. Biophys. Res. Commun. 187:590–595.
Samson, W. K., and Skala, K. D. (1992). Comparison of the pituitary effects of the mammalian endothelins: Vasoactive intestinal contractor (endothelin-β, rat endothelin-2) is a potent inhibitor of prolactin secretion. Endocrinology 130:2964–2970.
Sato, T., Niwa, M., Himeno, A., Tsutsumi, K., and Amemiya, T. (1993). Quantitative receptor autoradiographic analysis for angiotensin II receptors in bovine retinal microvessels: Quantitation with radioluminography. Cell. Mol. Neurobiol. 13:233–245.
Shichiri, M., Hirata, Y., Nakajima, T., Ando, K., Imai, T., Yanagisawa M., Masaki, T., and Marumo, F. (1991). Endothelin-1 is an autocrine/paracrine growth factor for human cancer cell lines. J. Clin. Invest. 87:1867–1871.
Shuangshoti, S., Netsky, M. G., and Nashold, B. S. (1970). Epithelial cysts related to sella turcica: proposed origin from neuroepithelium. Arch. Pathol. Lab. Med. 90:444–450.
Stojilković, S. S., and Catt, K. J. (1992). Neuroendocrine actions of endothelins. Trends Pharmacol. Sci. 13:385–391.
Stojilković, S. S., Merelli, F., Iida, T., Krsmanović, L. Z., and Catt, K. L. (1990). Endothelin stimulation of cytosolic calcium and gonadotropin secretion in anterior pituitary cells. Science 248:1663–1666.
Stojilković, S. S., Balla, T., Fukuda, S., Češnjaj, M., Merelli, F., Krsmanović, L. Z., and Catt, K. J. (1992). Endothelin ETA receptors mediate the signaling and secretory actions of endothelins in pituitary gonadotrophs. Endocrinology 130:465–474.
Takahashi, K., Ghatei, M. A., Jones, P. M., Murphy, J. K., Lam, H.-C., O'Halloran, D. J., and Bloom, S. R. (1991). Endothelin in human brain and pituitary gland: Presence of immunoreactive endothelin, endothelin messenger ribonucleic acid, and endothelin receptors. J. Clin. Endocrinol. Metab. 72:693–699.
Tsutsumi, K., Niwa, M., Kitagawa, N., Yamaga, S., Anda, T., Himeno, A., Sato, T., Khalid, H.,Taniyama, K., and Shibata, S. (1994). Enhanced expression of an endothelin ETA receptor incapillaries from human glioblastoma: a quantitative receptor autoradiographic analysis using aradioluminographic imaging plate system. J. Neurochem. 63:2240-2247.
Tsutsumi, K., Niwa, M., Kitagawa, N., Yamaga, S., Anda, T., Himeno, A., Sato, T., Khalid, H., Taniyama, K., and Shibata, S. (1994). Enhanced expression of an endothelin ETA receptor in capillaries from human glioblastoma: a quantitative receptor autoradiographic analysis using a radioluminographic imaging plate system. J. Neurochem. 63:2240–2247.
Williams, D. L., Jr., Jones, K. L., Pettibone, D. J., Lis, E. V., and Clineschmidt, B. V. (1991). Sarafotoxin S6c: an agonist which distinguishes between endothelin receptor subtypes. Biochem. Biophys. Res. Commun. 175:556–561.
Yamamoto, S., Inenaga, K., Kannan, H., Eto, S., and Yamashita, H. (1993). The actions of endothelin on single cells in the anteroventral third ventricular region and supraoptic nucleus in rat hypothalamic slices. J. Neuroendocrinol. 5:427–434.
Yamashita, K., Niwa, M., Kataoka, Y., Shigematsu, K., Himeno, A., Tsutsumi, K., Nakano-Nakashima, M., Sakurai-Yamashita, Y., Shibata, S., and Taniyama, K. (1994). Microglia with an endothelin ETB receptor aggregate in rat hippocampus CA1 subfields following transient forebrain ischemia. J. Neurochem. 63:1042–1051.
Yanagisawa, M., Kurihara, H., Kimura, S., Tomobe, Y., Kobayashi, M., Mitsui, Y., Yazaki, Y., Goto, K., and Masaki, T. (1988). A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shibata, S., Himeno, A., Shigematsu, K. et al. Endothelin Receptors in Rat Pituitary Gland. Cell Mol Neurobiol 17, 89–100 (1997). https://doi.org/10.1023/A:1026381105170
Issue Date:
DOI: https://doi.org/10.1023/A:1026381105170