Abstract
Purpose : In this study, we investigated the relationship between functionality of the acrosome and sperm morphology.
Methods : Acrosome reaction (AR) was separately determined in live and dead sperm and in those with normal, small, and large sized acrosomes by means of the triple stain. Morphology was analyzed according to strict criteria after Papanicolaou stain.
Results : AR and morphology correlated regarding detection of large and small sized acrosomes, but not for normal sized acrosomes. Spontaneous AR was significantly influenced by acrosomal size. Sperm with large (11.4%) and normal (9.2%) acrosomes exhibited a significantly higher percentage of life spontaneously acrosome-reacted sperm than those with small acrosomes (4.5%). Sperm with small acrosomes were associated with a higher percentage of cell death.
Conclusion : The results indicate that sperm with small acrosomes are more susceptible to cell death and nonphysiological acrosomal loss. Acrosome size reflects the physiological capability of sperm function and therefore male fertility potential.
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REFERENCES
Yanagimachi R: Mechanisms of fertilization in mammals. In Fertilization and Embryonic Development In Vitro, L. Mastroianni, JD, Biggers (eds), New York, Plenum, 1981, pp 81–182
Suarez SS, Wolf DP, Meizel S: Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid. Gamete Res 1986;14:107–121
Blackmore PF, Beebe SJ, Danforth DR, Alexander N: Progesterone and 17α-hydroxyprogesterone. Novel stimulators of calcium influx in human sperm. J Biol Chem 1990;265:1376–1380
Franken DR, Bastiaan HS, Kidson A, Wranz P, Habenicht UF: Zona pellucida mediated acrosome reaction and sperm morphology. Andrologia 1997;29:311–317
Jamil K, White G: Induction of the acrosome reaction with ionophore A23187 and calcium. Arch Androl 1981;7:283–292
Sánchez R, Töpfer-Petersen E, Aitken RJ, Schill W-B: A new method for evaluation of the acrosome reaction in viable human spermatozoa. Andrologia 1991;23:197–203
Henkel R, Müller C, Miska W, Gips H, Schill, W-B: Determination of the acrosome reaction of human spermatozoa is predictive of fertilization in vitro. Hum Reprod 1993;8:2128–2132
Henkel R, Müller C, Miska W: Induction of acrosome reaction by low temperature is comparable to physiological induction by human follicular fluid. Andrologia 1998;30:159–161
Koehler JK, De Curtis I, Stechener, MA, Smith D: Interaction of human sperm with zona free hamster eggs. A freeze fracture study. Gamete Res 1982;6:371–386
Esterhuizen AD, Franken DR, Lourens JGH, van Rooyen LH: Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF. Hum Reprod 2001;16:138–144
Schill W-B: Some disturbances of acrosomal development and function in human spermatozoa. Hum Reprod 1991;6:969–978
Menkveld R, Stander FSH, Kotze TJvW, Kruger TF, Van Zyl JA: The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod 1990;5:586–592
Van Zyl JA, Kotze TJvW, Menkveld R: Predictive value of spermatozoa morphology in natural fertilization. In Human Spermatozoa in Assisted Reproduction, AA Acosta, RJ Swanson, SB Ackerman, TF Kruger, JA Van Zyl, R Menkveld (eds), Baltimore, Williams and Wilkins, 1990, pp 319–324
Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JC, Van Zyl JA, Smith K: Sperm morphology as a prognostic factor in in vitro fertilization. Fertil Steril 1986;46:1118–1126
Toner JP, Massad H, Grow DR, Moreshdy M, Swanson RJ, Oehninger S: Value of sperm morphology assessed by strict criteria for prediction of the outcome of artificial (intrauterine) insemination. Andrologia 1995;27:143–148
Coetzee K, Kruger TF, Lombard CJ: Predictive value of normal sperm morphology: A structured literature review. Hum Reprod Update 1998;4:73–82
Franken DR, Smith M, Menkveld R, Kruger TF, Kigondu C, Mbizvo M, Akanda EO: The development of a continuous quality control programme for strict sperm morphology among sub-Saharan African laboratories. Hum Reprod 2000;15:667–671
Menkveld R, Franken DR, Kruger TF, Oehninger S, Hodgen GD: Sperm selection capacity of the human zona pellucida. Mol Reprod Dev 1991;30:346–352
Liu DY, Baker HWG: Morphology of spermatozoa bound to the zona pellucida of human oocytes that failed to fertilize in vitro. J Reprod Fert 1992;94:71–84
Liu DY, Baker HWG: The proportion of human sperm with poor morphology but normal intact acrosomes detected with pisum sativum agglutinin correlates with fertilization in vitro. Fertil Steril 1988;50:288–293
Menkveld R, Rhemrev JPT, Franken DR, Vermeiden JPW, Kruger TF: Acrosomal morphology as a novel criterion for male fertility diagnosis: Relation with acrosin activity, morphology (strict criteria), and fertilization in vitro. Fertil Steril 1996;65:637–644
Talbot P, Chacon R: Triple stain technique for evaluating normal acrosome reactions of human sperm. J Exp Zool 1981;215:201–208
Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S: Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988;49:112–117
Burkman LJ, Coddington CC, Franken DR, Kruger TF, Rosenwaks Z, Hodgen GD: The hemizona assay (HZA): Development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 1988;49:688–697
Tesarik J: Acrosome reaction and testing. In Consensus Workshop on Advanced Diagnostic Andrology Techniques.ESHRE Andrology Special InterestGroup. Hum Reprod 1996;11:1467–1470
Schill W-B, Henkel R: Advancement in biochemical assays in andrology. Asian J Androl 1999;1:45–51
Contreras HR, Llanos MN: Detection of progesterone receptors in human spermatozoa and their correlation with morphological and functional properties. Int J Androl 2001;24:246–252
Carrell DT, Zobell RL, Middleton RG, Urry RL: A functional analysis and the potential clinical significance of 7 categories of sperm morphology. J Urol 1994;151:376–380
Heywinkel E, Freundl G, Hofmann N: Acrosome reaction of spermatozoa with different morphology. Andrologia 1993;25:137–139
Henkel R, Franken DR, Habenicht UF: Zonapellucida as physiological trigger for the induction of acrosome reaction. Andrologia 1998;30:275–280
Menkveld R, Wong WY, Lombard CJ, Wetzels AMM, Thomas CMG, tMerkus HMWM, Steegers-Theunissen PM: Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: An effort towards standardization of in-vivo thresholds. Hum Reprod 2001;16:1165–1171
Menkveld R, Stander FSH, Kruger TF: Comparison between the acrosome index and teratozoospermia index as additional criteria to sperm morphology in the prediction of expected invitro fertilization outcome. Hum Reprod 1998;13:52
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Menkveld, R., El-Garem, Y., Schill, WB. et al. Relationship Between Human Sperm Morphology and Acrosomal Function. J Assist Reprod Genet 20, 432–438 (2003). https://doi.org/10.1023/A:1026288710638
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DOI: https://doi.org/10.1023/A:1026288710638