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Construction of an amylolytic industrial strain of Saccharomyces cerevisiae containing the Schwanniomyces occidentalis α-amylase gene

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Abstract

The gene encoding Schwanniomyces occidentalis α-amylase (AMY) was introduced into the chromosomal δ sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an δ-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AMY gene and aureobasidin A resistance gene (AUR1-C) as the selection marker. The AMY gene was expressed under the control of the alcohol dehydrogenase gene promoter (ADC1p). The α-amylase activity of Sacc. cerevisiae transformed with this integrative cassette was 6 times higher than that of Sch. occidentalis. The transformants (integrants) were mitotically stable after 100 generations in nonselective medium.

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Authors and Affiliations

  1. Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University, Gwangju, 500-757, Korea

    Na-Young Kang, Jeong-Nam Park, Hwanghee Blaise Lee, Suhn-Young Im & Suk Bai

  2. Department of Cosmetology, Dongkang College, Gwangju, 500-714, Korea

    Jong-Eon Chin

Authors
  1. Na-Young Kang
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  2. Jeong-Nam Park
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  3. Jong-Eon Chin
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  4. Hwanghee Blaise Lee
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  5. Suhn-Young Im
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  6. Suk Bai
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Correspondence to Suk Bai.

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Kang, NY., Park, JN., Chin, JE. et al. Construction of an amylolytic industrial strain of Saccharomyces cerevisiae containing the Schwanniomyces occidentalis α-amylase gene. Biotechnology Letters 25, 1847–1851 (2003). https://doi.org/10.1023/A:1026281627466

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