Abstract
Cyclomaltoheptaose (β-cyclodextrin, β-CD) is a promising compound for application in various industrial fields because of its ability to entrap various compounds into its hydrophobic cavity. A monoclonal antibody (A7) to β-CD was generated by using a conjugate of glucosaminylmaltosyl-β-CD and bovine serum albumin as an antigen. The A7 monoclonal antibody was IgM/κ and reacted with β-CD with high specificity. The epitope recognized by the A7 monoclonal antibody seemed to be located on the secondary hydroxyl groups of the rim side of the β-CD molecule. The dissociation constant of the complex of β-CD and the immobilized A7 monoclonal antibody was determined to be 1.2 × 10-4 M. A competitive ELISA using the A7 monoclonal antibody enabled determination of β-CD and its derivatives with a detection limit of 0.05 μM. This immunoassay was useful to determine β-CD in biological fluids such as human plasma and urine after appropriate pretreatment of the samples.
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Ikura, K., Fujimoto, Ji., Kubonishi, K. et al. A monoclonal antibody to cyclomaltoheptaose (β-cyclodextrin): Characterization and use for immunoassay of β-cyclodextrin and its derivatives. Cytotechnology 40, 23–29 (2002). https://doi.org/10.1023/A:1023997501517
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DOI: https://doi.org/10.1023/A:1023997501517