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Identification of ribosomal protein S3a as a candidate for a novel PI 3-kinase target in the nucleus

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Abstract

Phosphatidylinositol 3,4,5-trisphosphate (PIP3) is an important lipid second messenger that mediates various cell responses. We have searched for the nuclear PIP3 binding proteins using PIP3 analogue beads. A 33 kD protein was detected in this method, which was identified as ribosomal protein S3a by the mass spectrometric analysis. The recombinant S3a protein bound specifically to PIP3. S3a localized not only in the cytosol but also in the nucleus. Interestingly, not cytosolic but nuclear S3a bound to PIP3, suggesting different roles of S3a in the cytosol and the nucleus.

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Authors and Affiliations

  1. Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan

    Norihisa Hamaguchi, Tsutomu Ohdaira, Sayoko Ihara & Yasuhisa Fukui

  2. Kirin Brewery, Fukuura Kanazawa-ku Yokohama City, Kanagawa, Japan

    Azusa Shinohara & Akihiro Iwamatsu

Authors
  1. Norihisa Hamaguchi
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  2. Tsutomu Ohdaira
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  3. Azusa Shinohara
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  4. Akihiro Iwamatsu
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  5. Sayoko Ihara
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  6. Yasuhisa Fukui
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Correspondence to Yasuhisa Fukui.

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Hamaguchi, N., Ohdaira, T., Shinohara, A. et al. Identification of ribosomal protein S3a as a candidate for a novel PI 3-kinase target in the nucleus. Cytotechnology 40, 85–92 (2002). https://doi.org/10.1023/A:1023970222898

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  • DOI: https://doi.org/10.1023/A:1023970222898

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