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Microsupport with two-dimensional geometry (2D-MS) 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHex® (CryoHex)

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Abstract

Tissue culture flasks were activated by electron beam irradiation and subsequently treated with N-isopropylacrylamide to make them cryoresponsive. Leaving such ‘cryoflasks’ unattended for 10 minutes at room temperature sufficed to almost completely detach the anchorage-dependent cells. MicroHex®, a polystyrene-based tissue culture microsupport with two-dimensional geometry, was handled in the same way to obtain CryoHex, i.e. a cryoresponsive MicroHex from which anchorage-dependent cells could be detached by exposure to low temperature (4–20 °C). Experimental conditions were determined allowing one to detach the cells from small and large microsupport culture volumes. Cells detached from CryoHex by exposure to low temperature displayed a high cell viability and, upon subcultivation on MicroHex®, did not show any alteration of their growth kinetics.

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Authors and Affiliations

  1. 4C Biotech s.a., Rue de la Marlette 14, Seneffe, B-7180, Belgium

    N. Kenda-Ropson, S. Lenglois & A.O.A. Miller

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  1. N. Kenda-Ropson
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  2. S. Lenglois
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  3. A.O.A. Miller
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Kenda-Ropson, N., Lenglois, S. & Miller, A. Microsupport with two-dimensional geometry (2D-MS) 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHex® (CryoHex). Cytotechnology 39, 163–170 (2002). https://doi.org/10.1023/A:1023949716842

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  • DOI: https://doi.org/10.1023/A:1023949716842

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