Abstract
A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome patients and compared it with controls. The results show that the prevalent sequence of centromere separation was X, 13, 21, Y and 18 in Down syndrome patients and Y, 13, X, 21 and 18 in controls. Chromatid segregation in early anaphase was asynchronic for all chromosome pairs studied. Late anaphase showed a frequency of non-disjunction of 4.5% in the controls, affecting only chromosomes 18 and Y; in the Down syndrome patients, the frequency was higher (20.3%) and affected all chromosomes studied. This technique could be applicated to know the incidence of non disjunction in couples with repetitive abortions or in cases with different aneuploidies in the offspring.
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Escudero T., Fuster C., Coll M.D. and Egozcue J. 1998. Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization. Cancer Genet Cytogenet 100: 111-113.
Fitzgerald P.H. and McEwan C.M. 1987. Total aneuploidy and age-related sex chromosome aneuploidy in cultured lymphocytes of normal men and women. Hum Genet 39: 329-337.
Ford J. and Congedi M.M. 1987. Rapid induction of anaphase in competent cells by hypotonic treatment. Cytobios 51: 183-192.
Keser I., Lüleci G. and Gündüz G. 1996. Premature centromere division in three unrelated families. Ann Genet 398: 87-90.
Méhes K. and Kosztolányi G. 1992. A possible mosaic form of delayed centromere separation and aneuploidy. Hum Genet 88: 477-478.
Mitchinson T. and Salmon E.D. 1992. Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J Cell Biol 119: 569-582.
Vig B.K. and Rattner J.B. 1989. Centromere, kinetochrore, and cancer. Crit Rev Oncog 1: 343-371.
Zijno A., Leopardi P., Marcon F. and Crebelli R. 1996. Sex chromosome loss and non-disjunction in women: Analysis of chromosomal segregation in binucleated lymphocytes. Chromosoma 104: 461-467.
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Escudero, T., Coll, M., Fuster, C. et al. Chromatid segregation analysis in native human lymphocyte anaphases using sequential fluorescence in situ hybridization. Cytotechnology 39, 171–175 (2002). https://doi.org/10.1023/A:1023945218123
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DOI: https://doi.org/10.1023/A:1023945218123