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Inhibin B Predicts Oocyte Number and the Ratio IGF-I/IGFBP-1 May Indicate Oocyte Quality During Ovarian Hyperstimulation for In Vitro Fertilization

  • Gabriel Fried
  • Katarina Remaeus
  • Jonas Harlin
  • Elisabeth Krog
  • György Csemiczky
  • Arthur Aanesen
  • Michael Tally
Article

Abstract

Purpose: To perform a retrospective analysis of 62 age-matched IVF-treated women in order to investigate whether levels of inhibin B, IGF-I, and IGFBP-1 in serum 2 days before oocyte retrieval and in follicular fluid at the day of oocyte retrieval might be useful as indicators of the ovarian ability to produce oocytes (ovarian reserve).

Methods: Patients were allocated into three groups on the basis of the number of oocytes retrieved. Group 1 (“low responders”) had 0–3 oocytes, group 2 (“normal responders”) had 6–11 oocytes, and group 3 (“high responders”) had 12 oocytes or more. Levels of inhibin B, IGF-I, and IGFBP-1 in follicular fluid and in serum obtained 2 days before oocyte retrieval were analyzed and correlated to clinical parameters including estradiol levels, progesterone levels, follicle size, follicle number, and oocyte number.

Results: We found significant differences in inhibin B levels in the three groups. Inhibin B levels in follicular fluid and serum was strongly correlated to the number of oocytes retrieved (p < 0.01). The number of oocytes retrieved were also correlated to total FSH dose (p < 0.05), to estradiol 2 days before and at ovum pick-up (p < 0.05), to progesterone at ovum pick-up (p < 0.0001), to progesterone at embryo transfer (p < 0.05), and to the number of follicles (size 12–15 mm, p < 0.001, size>15 mm, p < 0.01). Serum inhibin B also correlated to follicular fluid inhibin B (p < 0.01). Inhibin B was not correlated to pregnancy. In contrast, the ratio IGF-I/IGFBP-1 in serum as well as in follicular fluid was significantly higher in women who became pregnant (p < 0.05).

Conclusions: The results show that inhibin B in serum 2 days before oocyte retrieval predicts number of oocytes retrieved. Since inhibin B in serum before oocyte retrieval in ovarian hyperstimulation was strongly predictive of the number of oocytes retrieved, it appears useful as a marker for ovarian response. Inhibin B did not predict treatment outcome, whereas the ratio IGF-I/IGFBP-1 in serum and follicular fluid was significantly higher in women who became pregnant. The ratio IGF-I/IGFBP-1 may thus reflect oocyte quality.

IGF-I IGFBP-1 inhibin IVF oocyte 

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Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Gabriel Fried
    • 1
  • Katarina Remaeus
    • 1
  • Jonas Harlin
    • 1
  • Elisabeth Krog
    • 2
  • György Csemiczky
    • 1
  • Arthur Aanesen
    • 1
  • Michael Tally
    • 2
  1. 1.Reproductive Medical Center, Department of Women and Child Health, Division of Obstetrics and GynecologyKarolinska HospitalStockholmSweden
  2. 2.Department of Molecular MedicineKarolinska InstituteStockholmSweden

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