Fertilization Potential of Human Sperm Is Correlated with Endogenous Platelet-Activating Factor Content
- 32 Downloads
Purpose: Platelet-activating factor (PAF) is a potent signaling phospholipid that is found in mammalian sperm and has a positive correlation with fertility. Whereas PAF is present in human sperm, there are no relational reports on its content and the cells fertilization potential. Therefore, the study objective was to determine if PAF content in capacitated-induced sperm is related to fertilization potential as determined by the sperm penetration assay (SPA).
Methods: Endogenous sperm lipids were measured for PAF content by a specific radioimmunoassay following insemination of zona pellucida-free hamster ova. Data were analyzed by regression analysis and Student's t test.
Results: Regression analysis revealed a positive and significant relation (R2 = 0.806; P < 0.05) between PAF content in human sperm and SPA outcome (pass: ≥5.0; fail: <5.0, penetrations/ova). Patients that passed (22.61 ± 5.21 picomoles/106) the SPA had significantly (P < 0.01) higher PAF levels in their sperm than patients that failed (12.91 ± 1.76 picomoles/106 cells) the test.
Conclusions: PAF content in capacitated-induced sperm has a significant and positive relationship with fertilization potential. Fertilization potential may be predicted by measuring PAF levels in capacitation-induced human sperm. Determining PAF content in capacitated human sperm may be a beneficial diagnostic tool for the infertility specialist.
Unable to display preview. Download preview PDF.
- 7.LetendreED, MironP, RobertsKD, LanglaisJ: Platelet-activating factor acetylhydrolase in seminal plasma. Fert Steril 1992;57:193–198Google Scholar
- 8.GujaratiVR, NaukamRJ, Rama-SastryBV: Enzymatic deacetylation and acetylation of ether phospholipids related to platelet-activating factor in human semen with short and long liquefaction times. Ann NY Acad Sci 1987;513:583–585Google Scholar
- 14.RoudebushWE, FukudaAI, MinhasBS: Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet-activating factor (PAF) treated sperm. J Assis: Reprod Genet 1993;10:91–94Google Scholar
- 15.Minhas, BS: Platelet-activating factor treatment of human spermatozoa enhances fertilization potential: Am J Obstet Gynecol 1993;4:1314–1317Google Scholar
- 16.AngleMJ, TomR, JarviK, McClureRD: Effect of platelet-activating factor (PAF) on human sperm–oocyte interactions. J Reprod Fertil 1989;98(2):541–548Google Scholar