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Fertilization Potential of Human Sperm Is Correlated with Endogenous Platelet-Activating Factor Content

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Abstract

Purpose: Platelet-activating factor (PAF) is a potent signaling phospholipid that is found in mammalian sperm and has a positive correlation with fertility. Whereas PAF is present in human sperm, there are no relational reports on its content and the cells fertilization potential. Therefore, the study objective was to determine if PAF content in capacitated-induced sperm is related to fertilization potential as determined by the sperm penetration assay (SPA).

Methods: Endogenous sperm lipids were measured for PAF content by a specific radioimmunoassay following insemination of zona pellucida-free hamster ova. Data were analyzed by regression analysis and Student's t test.

Results: Regression analysis revealed a positive and significant relation (R 2 = 0.806; P < 0.05) between PAF content in human sperm and SPA outcome (pass: ≥5.0; fail: <5.0, penetrations/ova). Patients that passed (22.61 ± 5.21 picomoles/106) the SPA had significantly (P < 0.01) higher PAF levels in their sperm than patients that failed (12.91 ± 1.76 picomoles/106 cells) the test.

Conclusions: PAF content in capacitated-induced sperm has a significant and positive relationship with fertilization potential. Fertilization potential may be predicted by measuring PAF levels in capacitation-induced human sperm. Determining PAF content in capacitated human sperm may be a beneficial diagnostic tool for the infertility specialist.

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References

  1. YanagimachiR, YanagimachiH, RogersBJ: The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human sperm. Biol Reprod 1976;15(4):471–476

    PubMed  Google Scholar 

  2. LevineAS, KortHI, ToledoAA, RoudebushWE: A review of the effect of platelet-activating factor on male reproduction and sperm function. J Androl 2002;23(4):471–476

    PubMed  Google Scholar 

  3. HarperMJK: Platelet-activating factor: A paracrine factor in preimplantation stages of development? Biol Reprod 1989;40:907–913

    PubMed  Google Scholar 

  4. MinhasBS, KumarR, RickerDD, RobertsonJL, DodsonMG: The presence of platelet activating factor-like activity in human sperm. Fertil Steril 1991;55:372–376

    PubMed  Google Scholar 

  5. RoudebushWE, PurnellET: Platelet-activating factor content in human spermatozoa and pregnancy outcome. Fertil Steril 2000;74:257–260

    PubMed  Google Scholar 

  6. BlighEG, DyerWJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917

    PubMed  Google Scholar 

  7. LetendreED, MironP, RobertsKD, LanglaisJ: Platelet-activating factor acetylhydrolase in seminal plasma. Fert Steril 1992;57:193–198

    Google Scholar 

  8. GujaratiVR, NaukamRJ, Rama-SastryBV: Enzymatic deacetylation and acetylation of ether phospholipids related to platelet-activating factor in human semen with short and long liquefaction times. Ann NY Acad Sci 1987;513:583–585

    Google Scholar 

  9. KuzanFB, GeisslerFT, HendersonWR: Role of spermatozoal platelet-activating factor in fertilization. Prostaglandins 1990;39:61–74

    PubMed  Google Scholar 

  10. HellstromWJG, WangR, SikkaSC: Platelet-activating factor stimulates motion parameters of cryopreserved human sperm. Fertil Steril 1991;56:768–770

    PubMed  Google Scholar 

  11. WuC, StojanovT, ChamiO, IshiiS, ShimizuT, LiA, O'NeillC, ShimuzuT. Evidence for the autocrine induction of capacitation of mammalian spermatozoa. J Biol Chem 2001;276:26962–26968

    PubMed  Google Scholar 

  12. SengokuK, IshikawaM, TamateK, ShimizuT: Effects of platelet-activating factor on mouse sperm function. J Assist Reprod Genet 1992;9:447–453

    PubMed  Google Scholar 

  13. RoudebushWE, MinhasBS, RickerDD, PalmerTV, DodsonMG: Platelet activating factor enhances in vitro fertilization of rabbit oocytes. Am J Obstet Gynecol 1990;163:1670–1673

    PubMed  Google Scholar 

  14. RoudebushWE, FukudaAI, MinhasBS: Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet-activating factor (PAF) treated sperm. J Assis: Reprod Genet 1993;10:91–94

    Google Scholar 

  15. Minhas, BS: Platelet-activating factor treatment of human spermatozoa enhances fertilization potential: Am J Obstet Gynecol 1993;4:1314–1317

    Google Scholar 

  16. AngleMJ, TomR, JarviK, McClureRD: Effect of platelet-activating factor (PAF) on human sperm–oocyte interactions. J Reprod Fertil 1989;98(2):541–548

    Google Scholar 

  17. ReinhardtJC, CuiX, RoudebushWE: Immunofluorescent evidence for the presence of the platelet-activating factor receptor in human sperm. Fertil Steril 1999;71(5):941–942

    PubMed  Google Scholar 

  18. RoudebushWE, WildMD, MaguireE: Platelet-activating factor receptor expression in human sperm: Differences in mRNA content and protein distribution between normal and abnormal sperm. Fertil Steril 2000;73(5):967–971

    PubMed  Google Scholar 

  19. RoudebushWE: Role of platelet-activating factor in reproduction: Sperm function. Asian J Androl 2001;3:81–85

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia

    Andrew A. Toledo, Dorothy Mitchell-Leef, Carlene W. Elsner, Scott M. Slayden & William E. Roudebush

Authors
  1. Andrew A. Toledo
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  2. Dorothy Mitchell-Leef
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  3. Carlene W. Elsner
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  4. Scott M. Slayden
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  5. William E. Roudebush
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Toledo, A.A., Mitchell-Leef, D., Elsner, C.W. et al. Fertilization Potential of Human Sperm Is Correlated with Endogenous Platelet-Activating Factor Content. J Assist Reprod Genet 20, 192–195 (2003). https://doi.org/10.1023/A:1023622126870

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  • DOI: https://doi.org/10.1023/A:1023622126870

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