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Investigational New Drugs

, Volume 21, Issue 1, pp 15–20 | Cite as

Effect of the Antitumor Drug Vinblastine on Nuclear βII-tubulin in Cultured Rat Kidney Mesangial Cells

  • Consuelo Walss-Bass
  • Jeffrey I. Kreisberg
  • Richard F. Ludueña
Article

Abstract

Tubulin, the main component of microtubules, is a major target for antitumor drugs such as vinblastine. We have recently discovered that the βII isotype of tubulin is present in the nuclei of cultured rat kidney mesangial cells, smooth-muscle-like cells present in the renal glomerular mesangium (Walss C, Kreisberg JI, Ludueña RF: Cell Motil Cytoskeleton 42: 274–284, 1999). Here, we have investigated the effect of vinblastine on nuclear βII-tubulin in these cells. We have found that, at concentrations of 15 nM and higher, vinblastine caused a reversible loss of βII-tubulin from the nucleus. Our results raise the possibility that nuclear βII-tubulin constitutes a population of tubulin that could be a novel target for antitumor drugs such as vinblastine.

tubulin isotypes microtubules vinblastine nuclear localization DNA fragmentation 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Consuelo Walss-Bass
    • 1
  • Jeffrey I. Kreisberg
    • 2
  • Richard F. Ludueña
    • 1
  1. 1.Department of BiochemistryUniversity of Texas Health Science CenterSan AntonioUSA
  2. 2.Department of SurgeryUniversity of Texas Health Science CenterSan AntonioUSA

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