Hundreds of misleading reports are published every year containing data on human cancer cell lines that are derived from some other species, tissue or individual to that claimed. In consequence, millions of dollars provided for cancer research are being spent on the production of misleading data. This review describes how cross-contamination occurs, catalogues the use of false cell lines in leading biomedical journals, and suggests ways to resolve the problem.
Unable to display preview. Download preview PDF.
- Chakraborty M, Qiu SG, Vasudevan KM and Rangnekar VM(2001) Par-4 drives trafficking and activation of Fas and FasL to induce prostate cancer cell apoptosis and tumor regression. Cancer Res 61: 7235–7263.Google Scholar
- Dirks WG, MacLeod RA and Drexler HG (1999) ECV304 (endothelial) is really T24 (bladder carcinoma): Cell line crosscontamination at source. In Vitro Cell Devl Biol 35: 558–559.Google Scholar
- Editorial (1981) Responsibility for trust in research. Nature 289: 211–212.Google Scholar
- Gey GO, Coffman WD and Kubicek MT (1952) Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res 12: 264–265.Google Scholar
- Gold M (1986) A conspiracy of cells. One woman's immortal legacy and the scandal it caused. State University of New York Press.Google Scholar
- Masters JRW, Thomson JA, Daly-Burns B, Reid YA, Dirks W, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KG and Debenham PG (2001) STR profiling provides an international reference standard for human cell lines. Proc Natl Acad Sci USA 98: 8012–8017.PubMedCrossRefGoogle Scholar
- Merten O-W (1989) Culture of Hybridomas - A Survey. In: Miller AOA (ed.) Advanced Research on Animal Cell Technology, NATO ASI Series E. Applied Sciences, Vol. 156, (pp. 367–398) Kluwer Academic Publishers, Dordrecht.Google Scholar