Abstract
Purpose: Our purpose was to detect aneuploidy for chromosomes 13, 16,18, 21, 22, X, and Y in preimplantation embryos from patients with a history of unexplained recurrent miscarriage.
Methods: Three patients with a history of unexplained recurrent spontaneous abortion were included in this study. Embryos were biopsied at the eight-cell stage, individually fixed on slides, and processed for fluorescent in situ hybridization (FISH). A multiple FISH protocol for seven chromosomes pairs (13, 16, 18, 21, 22, X, and Y) has been developed.
Results: A total of 39 embryos was studied with the multiple FISH protocol developed. Successful analysis of the biopsied embryos was achieved within the time limits usually allowed in a preimplantation diagnosis program. Analysis of the blastomeres showed that 17 embryos were chromosomally normal for the probes used, 16 embryos were aneuploid, and in 6 embryos no informative results were obtained.
Conclusions: In the patients studied, a large proportion of embryos (41%) exhibited chromosomal abnormalities for the probes used. Preimplantation diagnosis to screen for chromosome abnormalities could be a feasible approach to improve the possibility of successful pregnancy in these couples.
Similar content being viewed by others
REFERENCES
Stirrat G. Recurrent miscarriage I. Definition and epidemiology. Lancet 1990;336:673-675
Berry CW, Brambati B, Eskes TKAB, Exalto N, Fox H, Geraedts JPM, Gerhart I, Gonzales Gomes F, Grudzinskas JG, Hustin J, Jouppila P, Lindblom, Mantoni M, Montenegro N, Nogales Fernandes F, O'Rahilly R, Pedersen JF, Peters PWJ, Regan L, Rushton DI, van Straaten HWM, Tarlatzis BC, Wells M: The Euro-team early pregnancy (ETEP) protocol for recurrent miscarriage. Hum Reprod 1995;10:1516-1520
Simón C, Gutierrez A, Vidal A, de los Santos MJ, Tarín JJ, Remohí J, Pellicer A: Outcome of patients with endometriosis in assisted reproduction: Results from in vitro fertilization and oocyte donation. Hum Reprod 1994;9:725-729
Balasch J, Creus M, Fábregues F, Cívico S, Carmona F, Martorell J, Vanrell JA: In vitro fertilization treatment for unexplained recurrent abortion: A pilot study. Hum Reprod 1996;11:1579-1582
Hassold T: Chromosome abnormalities in human reproductive wastage. Trends Genet 1978;2:105-110
Hasold TJ, Jacobs PA: Trisomy in man. Annu Rev Genet 1984;18:69-97
Brambati B: Fate of human pregnancies. In Establishing a Successful Human Pregnancy, RG Edwards (ed). New York, Raven Press, 1990, pp 269-281
Eiben B, Bartels I, Bähr-Porsch S, Borgmann S, Gatz G, Gellert G, Goebel R, Hammans W, Hentemann M, Osmers R, Rauskolb R, Hansmann I: Cytogenetic analysis of 750 spontaneous abortions with the direct-preparation method of chorionic and its implications for studying genetic causes of pregnancy wastage. Am J Hum Genet 1990;47:656-663
Egozcue J: Of course not. Hum Reprod 1996;11:2077-2078
Tarkowski AK: An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 1966;5:394-400
Munné S, Lee A, Rosewaks Z, Grifo J, Cohen J: Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum Reprod 1993;8:2185-2191
Benadiva CA, Kligman I, Munné S: Aneuploidy 16 in human embryos increases significantly with maternal age. Fertil Steril 1996;66:248-255
Escudero T, Coll MD, Fuster C, Egozcue J: Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization. Cancer Genet Cytogenet 1998;100:111-113
Munné S, Alikani M, Tomkin G, Grifo J, Cohen J: Embryo morphology, developmental rates and maternal age are correlated with chromosome abnormalities. Fertil Steril 1995;64:382-391
Egozcue J, Templado C, Vidal F, Navarro J, Morer-Fargas F, Marina S: Meiotic studies in a series of 1100 infertile and sterile males. Hum Genet 1983;65:185-188
Plachot M: Oocyte-genetic aspects. In Gametes. The Oocyte, JG Grudzinkskas, IL Yovich (eds). Cambridge, Cambridge University Press, 1995, pp 95-107
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vidal, F., Giménez, C., Rubio, C. et al. FISH Preimplantation Diagnosis of Chromosome Aneuploidy in Recurrent Pregnancy Wastage. J Assist Reprod Genet 15, 310–313 (1998). https://doi.org/10.1023/A:1022552713015
Issue Date:
DOI: https://doi.org/10.1023/A:1022552713015