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Autocrine Effects of Transforming Growth Factor-α on the Development of Preimplantation Mouse Embryos

  • Yoshimasa Onohara
  • Tasuku Harada
  • Masahiro Tanikawa
  • Tomio Iwabe
  • Hiroki Yoshioka
  • Fuminori Taniguchi
  • Masahiro Mitsunari
  • Toshiyuki Tsudo
  • Naoki Terakawa
Article

Abstract

Purpose: We wished to explore the role of transforming growth factor (TGF)-α in mouse embryonic development.

Methods: We examined the gene expression of TGF-α and epidermal growth factor receptor (EGFR) in mouse blastocysts by the reverse transcription–polymerase chain reaction and evaluated the effects of TGF-α on the development of preimplantation mouse embryos using TGF-α antisense oligodeoxynucleotide. Mouse teratocarcinoma F9 cells were also a subject of this study.

Results: Gene transcripts of TGF-α and EGFR were present in both blastocysts and F9 cells. TGF-α significantly stimulated the rate of blastocoel expansion in early-cavitating blastocysts and the proliferation of F9 cells. Northern blot analysis showed that TGF-α gene expression in F9 cells was markedly suppressed in the presence of TGF-α antisense oligodeoxynucleotide. TGF-α antisense oligonucleotide significantly reduced the rate of blastocoel expansion and the growth of F9 cells. The inhibitory effects of TGF-α antisense oligonucleotide on blastocysts and F9 cells were reversed by the addition of TGF-α.

Conclusions: The present observations suggest that TGF-α acts as an autocrine factor in the development of preimplantation mouse embryos.

transforming growth factor-α epidermal growth factor receptor preimplantation embryo teratocarcinoma F9 cell antisense oligodeoxynucleotide 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Yoshimasa Onohara
    • 1
  • Tasuku Harada
    • 1
  • Masahiro Tanikawa
    • 1
  • Tomio Iwabe
    • 1
  • Hiroki Yoshioka
    • 1
  • Fuminori Taniguchi
    • 1
  • Masahiro Mitsunari
    • 1
  • Toshiyuki Tsudo
    • 1
  • Naoki Terakawa
    • 1
  1. 1.Department of Obstetrics and GynecologyTottori University School of MedicineYonagoJapan

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