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Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe

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Abstract

We report here the first functional over-expression of the Stm1 protein, a G-protein-coupled receptor with seven-trans-membrane spanning regions, in a homologous expression system without internal modification of the open reading frame of Stm1. The entire coding sequence, except for the termination codon followed by a C-terminal His6 tag, has been cloned into the pREP1 vector. The functionally active Stm1-His6 was over-expressed in Schizosaccharomyces pombe under the control of the nmt1 (no message in thiamine) promoter. The expression after induction was 120 times as much as that of control before induction and it gave ∼500 ng protein/2 × 107cells.

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Authors and Affiliations

  1. Division of Genomics & Proteomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea

    Kyung-Sook Chung, Dong-Uk Kim, Sung-Woo Ryoo, Eun-Jung Kang, Misun Won, Lila Kim, Young-Joo Jang, Hyang-Sook Yoo & Kwang-Lae Hoe

  2. Department of Microbiology, Chungnam National University, Yusong, Taejon, Korea

    Pil-Jae Maeng

  3. Department of Biology & Medicinal Science, Paichai University, Taejon, Korea

    Sei-Chang Kim

Authors
  1. Kyung-Sook Chung
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  2. Dong-Uk Kim
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  3. Sung-Woo Ryoo
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  4. Eun-Jung Kang
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  5. Misun Won
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  6. Lila Kim
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  7. Young-Joo Jang
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  8. Pil-Jae Maeng
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  9. Sei-Chang Kim
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  10. Hyang-Sook Yoo
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  11. Kwang-Lae Hoe
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Corresponding author

Correspondence to Kwang-Lae Hoe.

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Chung, KS., Kim, DU., Ryoo, SW. et al. Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe . Biotechnology Letters 25, 267–272 (2003). https://doi.org/10.1023/A:1022355102192

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  • DOI: https://doi.org/10.1023/A:1022355102192

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