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Anti-Macrophage Inhibitory Factor Antibody Inhibits PMSG-hCG-Induced Follicular Growth and Ovulation in Mice

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Abstract

Purpose : To investigate the effect of an anti-MIF antibody on PMSG-hCG-induced murine follicular growth and ovulation and to determine whether MIF plays an essential role in this process.

Methods : Mice were primed with an intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and were treated with an anti-rat MIF antibody and human chorionic gonadotropin (hCG) to induce ovulation. After that, the ovulated ova were counted. The ovaries were studied using standard histological procedures.

Results : Ovaries treated with the anti-MIF antibody showed reduced numbers of growing follicles surrounded by granulosa cells and theca cells with a little proliferation compared with the control. The average numbers of ova collected from mice treated with the anti-MIF antibody were reduced compared with those collected from control mice.

Conclusions : Anti-MIF antibody inhibits the follicular growth and ovulation in mice, and MIF may play an important role in the inflammatory reactions during follicle growth and ovulation.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu-shi, Shizuoka, 431-3192, Japan

    Toshiki Matsuura, Motoi Sugimura, Takayuki Iwaki, Ryota Ohashi & Naohiro Kanayama

  2. Central Research Institute, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan

    Jun Nishihira

Authors
  1. Toshiki Matsuura
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  2. Motoi Sugimura
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  3. Takayuki Iwaki
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  4. Ryota Ohashi
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  5. Naohiro Kanayama
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  6. Jun Nishihira
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Correspondence to Toshiki Matsuura.

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Matsuura, T., Sugimura, M., Iwaki, T. et al. Anti-Macrophage Inhibitory Factor Antibody Inhibits PMSG-hCG-Induced Follicular Growth and Ovulation in Mice. J Assist Reprod Genet 19, 591–595 (2002). https://doi.org/10.1023/A:1021219317155

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  • DOI: https://doi.org/10.1023/A:1021219317155

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