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Cytotechnology

, Volume 38, Issue 1–3, pp 11–14 | Cite as

Over-expression of protein kinase Bα enhances recombinant protein expression in transient systems

  • Elham Ettehadieh
  • Sharon Wong-Madden
  • Teri Aldrich
  • Kelly Lane
  • Arvia E. MorrisEmail author
Article

Abstract

As more genes are being identified through genomic techniques,the need to rapidly express recombinant proteins for functionalstudies has become increasingly acute. Transient expression ofrecombinant protein using COS-1, CV-1 and 293 cells is widelyused to address this need. To improve the robustness of hostcells for transient expression, the effect of over-expression ofProtein Kinase Bα has been explored. In this report wedemonstrate that over-expression of Protein Kinase Bα canimprove transient recombinant protein expression 40% to >200%depending on the protein being expressed and the cell line used.

293 COS-1 and CV-1; PKBα>  recombinant protein transient expression 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Elham Ettehadieh
    • 1
  • Sharon Wong-Madden
    • 1
  • Teri Aldrich
    • 1
  • Kelly Lane
    • 1
  • Arvia E. Morris
    • 1
    Email author
  1. 1.Department of Cell SciencesImmunex CorporationSeattleUSA

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