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Cytotechnology

, Volume 38, Issue 1–3, pp 119–127 | Cite as

New technologies for automated cell counting based on optical image analysis `The Cellscreen'

  • Marlies Brinkmann
  • Dirk Lütkemeyer
  • Frank Gudermann
  • Jürgen Lehmann
Article

Abstract

A prototype of a newly developed apparatus for measuring cell growth characteristics of suspension cells in micro titre plates over a period of time was examined. Fully automated non-invasive cell counts in small volume cultivation vessels, e.g. 96 well plates, were performed with the Cellscreen system by Innovatis AG, Germany. The system automatically generates microscopic images of suspension cells which had sedimented on the base of the well plate. The total cell number and cell geometry was analysed without staining or sampling using the Cedex image recognition technology. Thus, time course studies of cell growth with the identical culture became possible. Basic parameters like the measurement range, the minimum number of images which were required for statistically reliable results, as well as the influence of the measurement itself and the effect of evaporation in 96 well plates on cell proliferation were determined. A comparison with standard methods including the influence of the cultured volume per well (25 μl to 200 μl) on cell growth was performed. Furthermore, the toxic substances ammonia, lactate and butyrate were used to show that the Cellscreen system is able to detect even the slightest changes in the specific growth rate.

automated cell counting Cedex cell concentration mammalian cells Trypan blue 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Marlies Brinkmann
    • 1
  • Dirk Lütkemeyer
    • 1
  • Frank Gudermann
    • 2
  • Jürgen Lehmann
    • 1
  1. 1.Institute of Cell Culture TechnologyUniversity of BielefeldBielefeldGermany
  2. 2.Innovatis AGBielefeldGermany

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