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Cytotechnology

, Volume 37, Issue 3, pp 171–178 | Cite as

Rapid detection of cytotoxicity of food additives and contaminants by a novel cytotoxicity test, menadione-catalyzed H2O2 production assay

  • Shiro Yamashoji
  • Kenji Isshiki
Article

Abstract

Menadione-catalyzed H2O2 production by viable animal cells was proportional to the viable cell number, and H2O2 production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotoxins, mycotoxins, and marine toxins were estimated using the above test employingNIH/3T3 and Neuro-2a cells. Synergistic effects of the toxin mixture were observed and acute cytotoxicity detected 1 h after the incubation of cells with toxins. This menadione-catalyzed H2O2production assay is rapid and simple compared to other popular cytotoxicity tests such as the MTT reduction assay and Neutral red inclusion test, requiring4 h. The menadione-catalyzed H2O2 production assay is expected to be a useful food safety test for rapidly detecting toxic compounds having a basic cytotoxic effect on common animal cells.

Cytotoxicity Food safety evaluation Menadione-catalyzed H2O2 production 

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References

  1. Ecobichon D.J. 1996. Toxic effects of pesticides. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 643–689.Google Scholar
  2. Ekwall B. 1983. Correlation between cytotoxicity in vitro and LD50values. Acta. Pharmacol. et. Toxicol. 52: 80–99.Google Scholar
  3. Goyer R.A. 1996. Toxic effects of metals. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 738.Google Scholar
  4. Ikemoto H. and Ueda S. 1994. Manual of Antibiotics and Infectious Disease. Hirokawa Publishing Co., Tokyo.Google Scholar
  5. Jaeger A., Jehl F. and Flesch F. 1993. Kinetics of amatoxins in human poisoning: Therapeutic implication. Clin. Toxicol. 31: 68–80.Google Scholar
  6. Kao C.Y. and Fuhrman F.A. 1963. Pharmacological studies on tarichatoxin, a potent neurotoxin. J. Pharmacol. Exp. Ther. 140: 31.Google Scholar
  7. Kenkens E.A.J., de Vrije T., van den Boom C., de Waard P., Plasman H.H., Thiel F. et al. 1995. Molecular basis of glycoal-kaloid induced membrane disruption. Biochim. Biophys. Acta. 1240: 216–228.CrossRefGoogle Scholar
  8. Konemann W.H. and Pieters M.N. 1996. Confusion of concepts in mixture toxicology. Food and Chem. Toxicol. 34: 1025–1031.CrossRefGoogle Scholar
  9. Kotsonis F.N., Burdock G.A. and Flamm W.G. 1996. Food toxicology. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 922.Google Scholar
  10. Manger R.L., Leja L.S., Lee S.Y., Hungerford J.M. and Wekell M.M. 1993. Tetrazolium-based cell bioassay for neurotoxins active on voltage-sensitive sodium channels: semiautomated assay for saxitoxins, brevetoxins, and ciguatoxins. Anal. Biochem. 214: 190–194.PubMedCrossRefGoogle Scholar
  11. Norton S. 1996a. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 848.Google Scholar
  12. Norton S. 1996b. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 842.Google Scholar
  13. Norton S. 1996c. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 849.Google Scholar
  14. Russel A.D. and Chopra I. 1990. Understanding Antibacterial Action and Resistance. Ellis Horwood Ltd., Publisher, Chichester, England.Google Scholar
  15. Van Zorge J.A. 1996. Exposure to mixtures of chemical substances: is there a need for regulation? Food and Chem. Toxicol. 34: 1033–1036.CrossRefGoogle Scholar
  16. Yamashoji S., Nishimoto F., Usuda F., Kubota H. and Isshiki K. 1992. Application of the chemiluminescent assay to cytotoxicity test: detection of menadione-catalyzed H2O2production by viable cells. Anal. Biochem. 207: 255–260.PubMedCrossRefGoogle Scholar
  17. Yamashoji S. and Isshiki K. 1998. Cytotoxicity testing for evaluating food safety. Altern. Anim. Test Exp. 5: 146–161.Google Scholar
  18. Yamashoji S. and Isshiki K. 1999. Novel cytotoxicity test based on menadione-catalyzed H2O2productivity for food safety evaluation. Cytotechnology 31: 45–51.CrossRefGoogle Scholar
  19. Yamazaki M., Nakajima T. and Fusetani N. 1995. Natutal Toxins. Kodansha, Tokyo.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Shiro Yamashoji
    • 1
  • Kenji Isshiki
    • 1
  1. 1.Kobe Gakuin Women's CollegeNagata-kuJapan

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