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Cytotechnology

, Volume 37, Issue 3, pp 143–151 | Cite as

Characterization of rainbow trout cell lines using microsatellite DNA profiling

  • G.M.L. Perry
  • G.J. McDonald
  • M.M. Ferguson
  • R.C. Ganassin
  • N.C. Bols
Article

Abstract

Ten microsatellite loci (Omy27DU,Omy325(A3)UoG, OmyFGT5TUF,OmyFGT14TUF, OmyFGT15TUF,OmyFGT23TUF, Omy77DU,Ssa20.19NUIG, Ots1BML, andOne18ASC) were amplified using the polymerase chain reaction to create genetic profiles for nine cell lines (RTG-2, RTH-149,RTL-W1,RTgill-W1, RTS-11, RTS-34st, RTP-2, RTP-91E and RTP-91F) from rainbow trout(Oncorhynchus mykiss) and one cell line (CHSE-214) from Chinook salmon (O. tschawytscha). A cell line (PHL) from anon-salmonid, the Pacific herring (Clupea harengus pallasi), was included as a control. The ten loci clearly revealed the uniqueness of each cell line, except for two cell lines (RTP-91E andRTP-91F) from the same fish. RTP-91E and RTP-91F were identical at all loci except Ssa20.19NUIG. The most useful locus for demonstrating uniqueness was Ots1BML. The information was used to demonstrate that an uncharacterized rainbow trout cell line (Clone 1A)was in fact CHSE-214, illustrating the usefulness of multiplexed microsatellites for the creation of genetic profiles for salmonid cell lines and for the testing of cell line cross-contamination.

Authentication DNA profiling Fish cell lines Microsatellite Rainbow trout 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • G.M.L. Perry
    • 1
  • G.J. McDonald
    • 1
  • M.M. Ferguson
    • 1
  • R.C. Ganassin
    • 2
  • N.C. Bols
    • 3
  1. 1.Department of ZoologyUniversity of GuelphGuelphCanada
  2. 2.Department of BiologyMalaspina University CollegeNanaimoCanada
  3. 3.Department of BiologyUniversity of WaterlooWaterlooCanada

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