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Journal of Assisted Reproduction and Genetics

, Volume 16, Issue 9, pp 488–491 | Cite as

Subpopulations of Human Granulosa–Luteal Cells Obtained from Gonadotropin- or Gonadotropin-Releasing Hormone Agonist/ Gonadotropin-Treated Follicles in In Vitro Fertilization–Embryo Transfer Cycles

  • Ksenija Gersak
  • Tomaz Tomazevic
Article

Abstract

Purpose: Our purpose was to find the differences in granulosa–luteal cells obtained from gonadotropin- versus gonadotropin-releasing hormone (GnRH) agonist/gonadotropin-treated follicles in in vitro fertilization–embryo transfer (IVF-ET) cycles.

Methods: Granulosa–luteal cells were obtained from 45 follicles of women undergoing IVF-ET with gonadotropin releasing hormone (GnRH) agonist and human menopausal gonadotropin (hMG) and from 45 follicles of women with hMG IVF-ET cycles. Subpopulations of granulosa–luteal cells were observed by computerized image analysis in which human chorionic gonadotropin (hCG) was localized using immunoperoxidase staining.

Results: The luteinized granulosa–luteal cells from hMG-treated follicles were larger than those from GnRH agonist/hMG-treated follicles. The hMG-treated follicles contained more hCG-stained cells, particularly those with cytoplasmic hCG localization.

Conclusions: We found differences in morphometric characteristics and hCG localization in granulosa–luteal cells obtained from hMG- versus GnRH agonist/hMG-treated follicles. We presume that the results indicate the influence and importance of luteal-phase support on the clinical pregnancy rate in GnRH agonist/hMG-treated IVF-ET cycles.

gonadotropin-releasing hormone agonist human granulosa–luteal cells human chorionic gonadotropin localization in vitro fertilization 

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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Ksenija Gersak
    • 1
  • Tomaz Tomazevic
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity Medical CenterSlajmerjeva 3Slovenia

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