Subpopulations of Human Granulosa–Luteal Cells Obtained from Gonadotropin- or Gonadotropin-Releasing Hormone Agonist/ Gonadotropin-Treated Follicles in In Vitro Fertilization–Embryo Transfer Cycles
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Purpose: Our purpose was to find the differences in granulosa–luteal cells obtained from gonadotropin- versus gonadotropin-releasing hormone (GnRH) agonist/gonadotropin-treated follicles in in vitro fertilization–embryo transfer (IVF-ET) cycles.
Methods: Granulosa–luteal cells were obtained from 45 follicles of women undergoing IVF-ET with gonadotropin releasing hormone (GnRH) agonist and human menopausal gonadotropin (hMG) and from 45 follicles of women with hMG IVF-ET cycles. Subpopulations of granulosa–luteal cells were observed by computerized image analysis in which human chorionic gonadotropin (hCG) was localized using immunoperoxidase staining.
Results: The luteinized granulosa–luteal cells from hMG-treated follicles were larger than those from GnRH agonist/hMG-treated follicles. The hMG-treated follicles contained more hCG-stained cells, particularly those with cytoplasmic hCG localization.
Conclusions: We found differences in morphometric characteristics and hCG localization in granulosa–luteal cells obtained from hMG- versus GnRH agonist/hMG-treated follicles. We presume that the results indicate the influence and importance of luteal-phase support on the clinical pregnancy rate in GnRH agonist/hMG-treated IVF-ET cycles.
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