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Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 10, pp 619–624 | Cite as

Isolation and Purification of an Early Pregnancy Factor–like Molecule from Culture Supernatants Obtained from Lymphocytes of Pregnant Women: II. Identification of the Molecule as a FC-Receptor–like Molecule: A Preliminary Report

  • Clara Aranha
  • Arvind Bordekar
  • Savitri Shahani
Article

Abstract

Purpose: Early pregnancy factor (EPF)–like activity from culture supernatants obtained from stimulated lymphocytes of pregnant women was characterized and identified.

Methods: The enzyme-linked immunosorbent assay depending on the presence of “Fc” receptors on bovine spermatozoa was used to identify the EPF-like molecule purified by gel filtration and reverse-phase high-performance liquid chromatography.

Results: The results indicated that the crude lymphocyte culture supernatant, the EPF-positive G IV fraction obtained on gel filtration, and the EPF-positive reverse-phase high-performance liquid chromatography protein readily bound with the different concentrations of aggregated human γ-globulin in a manner similar to that in which the standard control of aggregated human γ-globulin binds to the bovine spermatozoa.

Conclusions: EPF-like activity synthesized and secreted by lymphocytes during pregnancy may be a Fc-receptor–like molecule.

early pregnancy factor–like activity lymphocytes Fc-receptor–like molecule human γ-globulin 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Clara Aranha
    • 1
  • Arvind Bordekar
    • 1
  • Savitri Shahani
    • 1
  1. 1.Institute for Research in Reproduction (ICMR)Parel, MumbaiIndia

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