Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 10, pp 609–611 | Cite as

Centrifugal Pulsing Increases the Recovery of Viable Spermatozoa

  • Peter Bielfeld
  • Vida Acosta
  • R. S. Jeyendran


Purpose: Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.

Methods: A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk results determined the efficacy of the centrifugation techniques.

Results: The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.

Conclusions: Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.

pulse centrifugation viable spermatozoa 


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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Peter Bielfeld
    • 1
  • Vida Acosta
    • 2
  • R. S. Jeyendran
    • 2
  1. 1.Department of Obstetrics and GynecologyHeinrich Heine UniversityDusseldorfGermany
  2. 2.Andrology Laboratory ServicesChicago

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