Skip to main content
SpringerLink
Log in

Follicular Fluid Insulin-like Growth Factor-I and Insulin-like Growth Factor–Binding Protein-1 and -3 Vary as a Function of Ovarian Reserve and Ovarian Stimulation

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: Follicular fluid concentrations of insulin-like growth factor (IGF)-I, IGF-II, IGF-binding protein (BP)-1, and IGFBP-3 in 57 women undergoing in vitro fertilization and embryo transfer were examined to determine whether levels reflected differences in patients' exposure to gonadotropin stimulation and a diminished ovarian reserve.

Methods: Preovulatory follicular fluid was obtained from both gonadotropin-stimulated and unstimulated cycles. Subjects were grouped according to normal or decreased ovarian reserve and whether or not they received gonadotropin stimulation.

Results: The mean follicular fluid concentrations of IGF-I and IGFBP-1 were significantly lower in the “decreased” ovarian reserve group compared with the “normal” ovarian reserve group, with no change in estradiol or IGF-II levels. This resulted in a decreased molar IGF-I: BP ratio and an increased molar IGF-II:IGFBP-1 ratio. In unstimulated cycles, mean follicular fluid concentrations of IGFs did not differ significantly compared with those in stimulated cycles, whereas concentrations of IGFBP-1 and IGFBP-3 were significantly lower, leading to higher molar ratios of the IGFs to the binding proteins.

Conclusions: Follicular fluid IGF and binding proteins vary as a function of ovarian reserve and gonadotropin stimulation. This may reflect either differences in oocyte quality or a suboptimal follicular fluid environment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. Palermo GD, Munne S, Colomberg CT, Cohen J, Rosenwaks Z: Genetics of abnormal human fertilization. Hum Reprod 1995;10:120–127

    Google Scholar 

  2. Paulson RJ, Sauer MV, Lobo RA: Embryo implantation after human in vitro fertilization: Importance of endometrial receptivity. Fertil Steril 1990;53:870–874

    Google Scholar 

  3. Rogers PAW, Milne BJ, Trounson AO: A model to show human receptivity and embryo viability following ovarian stimulation for in vitro fertilization. J In Vitro Fert Embryo Transfer 1986;3:93–98

    Google Scholar 

  4. Geisthoevel F, Moretti-Rojas IM, Rojas FJ, Asch RH: Immunoreactive insulin-like growth factor I in human follicular fluid. Hum Reprod 1989;4:35–38

    Google Scholar 

  5. Adashi EY, Resnick CE, D'Ercole JS, Svoboda ME, Van Wyk JJ: Insulin-like growth factors as intraovarian regulators of granulosa cell growth and function. Endocrine Rev 1985;6:400–420

    Google Scholar 

  6. Hamori M, Blum WF, Torok A, et al.: Insulin-like growth factors and their binding proteins in human follicular fluid. Hum Reprod 1991;6:313–318

    Google Scholar 

  7. Baxter RC, Martin JL: Radioimmunoassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. J Clin Invest 1986;78:1504–1512

    Google Scholar 

  8. Giudice LC: Insulin-like growth factors and ovarian follicular development. Endocrine Rev 1992;13:641–669

    Google Scholar 

  9. Lee P, Conover CA, Powell DR: Regulation and function of insulin-like growth factor binding protein-1. Proc Soc Exp Biol Med 1993;204:4–29

    Google Scholar 

  10. El-Roeiy A, Chen X, Roberts VJ, LeRoith D, Roberts CT Jr, Yen SSC: Expression of insulin-like growth factor-I (IGF-I) and IGF II and the IGF-I, IGF-II, and insulin receptor genes and localization of the gene products is in the human ovary. J Clin Endocrinol Metab 1993;77:1411–1418

    Google Scholar 

  11. El-Roeiy A, Chen X, Roberts VJ, et al.: Expression of the genes encoding the insulin-like growth factors (IGF-I and II), the IGF and insulin receptors, and IGF-binding proteins-1-6 and the localization of their gene products in normal and polycystic ovary syndrome ovaries. J Clin Endocrinol Metab 1994;78:1488–1496

    Google Scholar 

  12. Eden JA, Jones J, Carter GD, Alaghband-Zadeh J: A comparison of follicular fluid levels of insulin-like growth factor-I in normal dominant and cohort follicles, polycystic and multicystic ovaries. Clin Endocrinol (Oxf) 1988;29:327–336

    Google Scholar 

  13. Van Dessel HJ, Chandrasekher YA, Yap OW, Lee PD, Hintz RL, Faessen GH, Braatt DD, Fauser BC, Giudice LC: Serum and Follicular fluid levels of insulin-like growth factor-I (IGF-I) IGF-II and IGF-binding protein-1 and-3 during the normal menstrual cycle. J Clin Endocrinol Metab 1996;81:122–126

    Google Scholar 

  14. Zhou J, Dsupin BA, Giudice LC, Bondy CA: Insulin-like growth factor system gene expression in human endometrium during the menstrual cycle. J Clin Endocrinol Metab 1994;79:1723–1734

    Google Scholar 

  15. Dor J, Ben-Shlomo I, Lunenfeld B, et al.: Insulin-like growth factor-I (IGF-I) may not be essential for ovarian follicular development: Evidence from IGF-I deficiency: J Clin Endocrinol Metab 1992;74:539–542

    Google Scholar 

  16. Jimena P, Castilla JA, Peran F, Molina, R, Ramirez JP, Acebal M, Vergara, F, Herruzo A: Insulin and insulin-like growth factor I in follicular fluid after induction of ovulation in women undergoing in vitro fertilization. J Reprod Fertil 1992;96:641–647

    Google Scholar 

  17. Artini PG, Battaglia C, D'Ambrogio G, Barreca, A, Droghini F, Volpe A, Genazzani AR: Relationship between human oocyte maturity, fertilization and follicular fluid growth factors. Hum Reprod 1994;9:902–906

    Google Scholar 

  18. Klein NA, Battaglia DE, Miller PB, Branigan EF, Giudice LC, Soules MR: Ovarian follicular development and the follicular fluid hormones and growth factors in normal women of advanced reproductive age. J Clin Endocrinol Metab 1996;81:1946–1951

    Google Scholar 

  19. Seifer DB, Giudice LC, Dsupin, BA, Haning RV Jr, Frishman GN, Burger HG: Follicular fluid insulin-like growth factor-I and insulin-like growth factor-II concentrations vary as a function of day 3 serum follicle stimulating hormone. Hum Reprod 1995;10:804–806

    Google Scholar 

  20. Blum WF, Ranke MB, Kietzmann K, Gauggel E, Zeiseel HHJ, Bierich J: A specific radioimmunoassay for the growth hormone (GH)-dependent somatomedin-binding protein: Its use for diagnosis of GH deficiency. J Clin Endocrinol Metab 1990;70:1292–1298

    Google Scholar 

  21. Juul A, Main K, Blum W, Lindholm J, Ranke M, Skakkebaek, N: The ratio between serum levels of insulin-like growth factor (IGF)-I and IGF binding proteins (IGFBP-1, 2 and 3) decreases with age in healthy adults and is increased in acromegalic patients. Clin Endocrinol 1994;41:85–93

    Google Scholar 

  22. Ibrahim ZH, Matson PL, Buck P, et al.: The use of biosynthetic human growth hormone to augment ovulation induction with buserelin acetate/human menopausal gonadotropin in women with a poor ovarian response. Fertil Steril 1991;55:202–204

    Google Scholar 

  23. Owen EJ, Shoham Z, Mason BA, et al.: Cotreatment with growth hormone, after pituitary suppression, for ovarian stimulation in vitro fertilization: a randomized, double-blind, placebo-control trial. Fertil Steril 1991;56:1104–1110

    Google Scholar 

  24. Hughes SM, Huang ZH, Morris ID, et al.: A double-blind cross-over controlled study to evaluate the effect of human biosynthetic growth hormone on ovarian stimulation in previous poor responders to in-vitro fertilization. Hum Reprod 1994;9:13–18

    Google Scholar 

  25. Bergh C, Hillensjo T, Wildand M, et al.: Adjuvant growth hormone treatment during in vitro fertilization: A randomized, placebo-controlled study. Fertil Steril 1994;62:113–201

    Google Scholar 

  26. Dor J, Sidman DS, Amudai E, et al.: Adjuvant growth hormone therapy in poor responders to in-vitro fertilization: Prospective randomized placebo-controlled double-blind study. Hum Reprod 1995;10:40–43

    Google Scholar 

  27. Conover CA, Powell DR: Insulin-like growth factor (IGF)-binding protein-3 blocks IGF-I-induced receptor down regulation and cell desensitization in cultured bovine fibroblasts. Endocrinology 1991;129:710–716

    Google Scholar 

  28. Eglin RG, Busby WH, Clemmons DR: An insulin-like growth factor (IGF) binding protein enhances the biologic response to IGF-I. Proc Natl Acad Sci USA 1988;4:3254–3259

    Google Scholar 

  29. Cataldo NA, Giudice LC: Insulin-like growth factor binding protein profiles in human ovarian follicular fluid correlate with follicular functional status. J Clin Endocrinol Metab 1992;74:821–829

    Google Scholar 

  30. Jones JL, Clemmons DR: Insulin-like growth factors and their binding proteins: biological actions. Endocrine Rev 1995:16:3–34

    Google Scholar 

  31. Hill DJ: Growth factors and the endocrine system. J Endocrinol 1987;115(Suppl):3

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, College of Physicians and Surgeons, Columbia University, Columbia Presbyterian Medical Center, New York, New York, 10032

    Laurel Stadtmauer, Andrea Vidali, Steven R. Lindheim & Mark V. Sauer

  2. North Carolina Center for Reproductive Medicine, Cary, North Carolina, 27511

    Laurel Stadtmauer

Authors
  1. Laurel Stadtmauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrea Vidali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Steven R. Lindheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark V. Sauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stadtmauer, L., Vidali, A., Lindheim, S.R. et al. Follicular Fluid Insulin-like Growth Factor-I and Insulin-like Growth Factor–Binding Protein-1 and -3 Vary as a Function of Ovarian Reserve and Ovarian Stimulation. J Assist Reprod Genet 15, 587–593 (1998). https://doi.org/10.1023/A:1020377209952

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020377209952

Search

Navigation