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Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 10, pp 605–608 | Cite as

Effects of Reducing Insemination Time in Human In Vitro Fertilization and Embryo Development by Using Sibling Oocytes

  • Serdar Coskun
  • Gayle L. Roca
  • Atif M. Elnour
  • Hend Al Mayman
  • Johannes M. G. Hollanders
  • Kamal A. Jaroudi
Article

Abstract

Purpose: Recent studies showed a beneficial effect of reducing the time of sperm–oocyte interaction on fertilization, division, and implantation rates of the oocytes obtained from randomized patients. In the present study, the effects of reduced insemination time on fertilization and embryo development were evaluated by using sibling oocytes from the same patient.

Methods: A total of 464 oocytes from 36 patients was randomly allocated to be inseminated for either 1 hr (reduced) or 18 hr (regular).

Results: Fertilization rates were not significantly different between reduced (135/229; 59%) and regular (150/235; 64%) groups. Cleavage rates and embryo quality were similar in both groups. A total of 135 embryos (73 from the reduced and 62 from the regular group) was transferred to 36 patients. Thirty-four embryos implanted in 18 patients (25.2% implantation and 50.0% pregnancy rates).

Conclusions: Fertilization, cleavage, and embryo development from 1-hr insemination is comparable, not superior, to those from an 18-hr insemination time, which is commonly used in in vitro fertilization programs. These data suggest that reduced insemination time can be used during in vitro fertilization to avoid unnecessarily longer exposure to spermatozoa.

embryo fertilization human in vitro fertilization reduced insemination 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Serdar Coskun
    • 1
  • Gayle L. Roca
    • 1
  • Atif M. Elnour
    • 1
  • Hend Al Mayman
    • 1
  • Johannes M. G. Hollanders
    • 2
  • Kamal A. Jaroudi
    • 2
  1. 1.Department of Pathology and Laboratory MedicineKing Faisal Specialist Hospital and Research CenterRiyadh 11211Saudi Arabia
  2. 2.Department of Obstetrics and GynecologyKing Faisal Specialist Hospital and Research CenterRiyadhSaudi Arabia

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