Low Blastocyst Formation Rates in Day-2 Fertilized Oocytes
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Purpose: To examine the blastocyst formation rates of day-2 fertilized oocytes.
Methods: A retrospective study of the outcomes/blastocyst formation of day-2 fertilized oocytes was undertaken.
Results: Fertilization rates of day-1 and -2 oocytes by intracytoplasmic sperm injection were similar. The development frequencies to four cells were similar. However, the blastulation rates were significantly lower from the day-2 fertilized eggs. The fertilization rates from day-2 conventional in vitro fertilization reinsemination were lower than the fertilization rates of day-1 oocytes. The blastulation rates from day-2 fertilized eggs were also lower than the rates from day-1 fertilized eggs in the in vitro fertilization group.
Conclusions: Fertilization is not a good indicator to predict the viability of fertilized oocytes. Day-2 fertilized oocytes had significantly lower blastocyst formation rates than the rates from day-1 fertilized oocytes.
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- 1.Trounson A, Webb J: Fertilization of human oocytes following reinsemination in vitro. Fertil Steril 1984;41:816–819Google Scholar
- 2.Boldt J, Howe AM, Butler WJ, McDonough PG, Padilla SL: The value of oocyte reinsemination in human in vitro fertilization. Fertil Steril 1987;48:617–623Google Scholar
- 3.Chen HL, Copperman AB, Grunfeld L, Sandler B, Bustillo M, Gordon JW: Failed fertilization in vitro: Second day micromanipulation of oocytes versus reinsemination. Fertil Steril 1995;63:1337–1340Google Scholar
- 4.Lundin K, Sjogren A: Reinsemination of one-day-old oocytes by use of intracytoplasmic injection. Fertil Steril 1996;66:118–121Google Scholar
- 5.Wun WSA, Wun CCC, Valdes CT, Dunn RC, Grunert GM: Optimal insemination concentration for human in vitro fertilization. Chinese J Physiol 1997;40:227–236Google Scholar
- 6.Van Steirteghem AC, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, Wisanto A, Devroey P: High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993;8:1061–1066Google Scholar
- 7.Almeida PA, Bolton VN: Immaturity and chromosomal abnormalities in occytes that fail to develop pronuclei following insemination in vitro. Hum Reprod 1993;8:229–232Google Scholar
- 8.Check JH, Long R, Allen J, Hoover L: Poor fertilization may be related to oocyte or zona pellucida recognition defects specific to certain hyperstimulation regimens and limited to some males but not others: A case report. Fertil Steril 1995;63:1341–1343Google Scholar
- 9.Wun W-S A, Wun C-C, Grunert GM: Minimum essential medium alpha enhances assisted reproductive technology results. I. mouse embryo study. J Assist Reprod Genet 1994;11:303–307Google Scholar
- 10.Lopata A, Hay DL: The surplus human embryo: Its potential for growth, blastulation, hatching, and human chorionic gonadotropin production in culture. Fertil Steril 1989;51:984–991Google Scholar
- 11.Muggleton-Harris AL, Glazier AM, Wall M: A retrospective analysis of the in-vitro development of “spare” human in-vitro fertilization preimplantation embryos using “in-house” prepared medium and “Medi-Cult” commercial medium. Hum Reprod 1995;10:2976–2984Google Scholar
- 12.Gardner DK, Vella P, Lane M, Wagely L, Schlenker, Schoolcraft WB: Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfer. American Society for Reproductive Medicine, 53rd Annual Meeting. Program Supplement, Cincinnati, OH, Oct 18–22, 1997 (abstr)Google Scholar
- 13.Wun WSA, Wun CC, Valdes CT, Dunn RC, Grunert GM: Blastocyst formation is a good indicator for attainment of assisted reproductive reproduction. Chinese J Physiol 1997;40:237–242Google Scholar
- 14.Eppig JJ, Schultz RM, O'Brien M, Chesnel F: Relationship between the development programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994;164:1–9Google Scholar