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Anti-inflammatory activity of superoxide dismutase conjugated with sodium hyaluronate

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Abstract

Superoxide dismutase (SOD) from bovine erythrocytes was conjugated with sodium hyaluronate (HA) with a mean molecular weight of 106 to have greater anti-inflammatory activity in vivo. Amino groups of SOD were coupled with carboxyl groups in the hyaluronate molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The HA-SOD conjugate was composed of 1.5 mol of SOD molecule per 1 mol of hyaluronate on the average, and retained 70% of the activity of unmodified SOD. The conjugate was essentially non-immunogenic in mice, and exhibited much higher anti-inflammatory activities than HA or SOD in models of inflammatory diseases such as ischemic oedema of the foot-pad in mice, carrageenin-induced pleurisy and adjuvant arthritis in rats.

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Authors and Affiliations

  1. Toin Human Science and Technology Centre, Department of Materials Science and Technology, Toin University of Yokohama, 1614 Kurogane-cho, Aoba-ku, Yokohama, 225, Japan

    Katsukiyo Sakurai, Yoh Kodera, Hiroyuki Nishimura & Yuji Inada

  2. The Tokyo Research Institute, Seikagaku Corporation, 3-1253 Tateno, Higashiyamato, Tokyo, 207, Japan

    Kyousuke Miyazaki

  3. Department of Internal Medicine, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, Beppu, 874, Japan

    Masao Shingu

Authors
  1. Katsukiyo Sakurai
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  2. Kyousuke Miyazaki
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  3. Yoh Kodera
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  4. Hiroyuki Nishimura
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  5. Masao Shingu
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  6. Yuji Inada
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Sakurai, K., Miyazaki, K., Kodera, Y. et al. Anti-inflammatory activity of superoxide dismutase conjugated with sodium hyaluronate. Glycoconj J 14, 723–728 (1997). https://doi.org/10.1023/A:1018521501289

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