Abstract
A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1–2 × 107 cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.
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Valle, M.A., Kester, M.B., Burns, A.L. et al. Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor. Cytotechnology 35, 127–135 (2001). https://doi.org/10.1023/A:1017586523710
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DOI: https://doi.org/10.1023/A:1017586523710