Abstract
A serum-free medium formulation – TUD-1 – was developed supporting growth of HUVEC in tissue culture. Special features of the basal medium formulation are highly elevated levels of glutamine and serine as well as the inclusion of N-acetylcysteine and phosphoascorbic acid. The cellular mitogenic needs are satisfied by bFGF, VEGF, EGF and liver growth factor. Further hormone supplementation consists of insulin and hydrocortisone. A protocoll for serum-free passage of HUVEC was established for serum-free long-term cultivation of freshly isolated HUVEC for up to 20 cumulative population doublings without significant differences in final cell density compared to controls cultivated with serum.
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Labitzke, R., Friedl, P. A serum-free medium formulation supporting growth of human umbilical cord vein endothelial cells in long-term cultivation. Cytotechnology 35, 87–92 (2001). https://doi.org/10.1023/A:1017551218007
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DOI: https://doi.org/10.1023/A:1017551218007