Abstract
Purpose: To demonstrate that human immature oocytes retrieved from women with regular menstrual cycles can undergo maturation and fertilization, and that the resulting embryos can establish pregnancies.
Methods: Immature oocytes (n = 568) were retrieved from women with regular menstrual cycle. The intact immature oocytes (n = 506) were allowed to mature in YS medium supplemented with 70% human follicular fluid (hFF); the matured oocytes were fertilized with husband sperm. Two pronuclei oocytes were cocultured with cumulus cells in YS medium supplemented with 10% hFF until 2 or 3 days after insemination. The cleaved embryos were transferred in uteri.
Results: Follicles were aspirated on Day 9.2 ± 5.3 of 63 natural cycles from 51 patients (mean age = 34.8 ± 4.0 years). The average number of retrieved immature oocytes was 9.0. The maturation rate was 74.3% (376/506). The two PN and cleavage rates were 72.6% (273/376) and 89.0% (243/273), respectively. Embryo transfer was achieved in 51 cycles and clinical pregnancy rate was 17.6% (9/51).
Conclusions: The results suggest that in vitro matured oocytes can undergo fertilization and the resulting embryos may successfully lead to pregnancies. However, further research is needed to improve IVM technique to achieve success rate comparable to gonadotrophin stimulated cycles.
Similar content being viewed by others
REFERENCES
Roest J, Mous HV, Zeilmaker GH, Verhoeff A: The incidence of major clinical complications in a Dutch transport IVF program. Hum Reprod Update 1996;2:345-353
Steward JA, Hamilton PJ, Murdoch AP: Thromboembolic disease associated with ovarian stimulation and assisted conception techniques. Hum Reprod 1997;12:2167-2173
Whittemore AS, Harris R, Itnyre J, and Collaborative Ovarian Cancer Group: Characteristics relating to ovarian cancer risk: Collaborative analysis of 12 US case-control studies. Am J Epidemiol 1992;136:1184-1203
Veeck LL, Wortham JW Jr, Witmyer J: Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 1983;39:594-602
Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Van Steirteghem A: Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: Case report. Fertil Steril 1996;65:1047-1050
Jaroudi KA, Hollanders JMG, Sieck UV, Roca GL, El-Nour AM, Coskun S: Pregnancy after transfer of embryos which were generated from in vitro-matured oocytes. Hum Reprod 1997;12:857-859
Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK: Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109-113
Trounson A, Wood C, Kausche A: In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62:353-362
Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO: Blastocyst development and birth after in vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995;10:3243-3247
Russell JB, Knezevich KM, Fabian KF, Dickson JA, MaCarthy CB: Unstimulated immature oocyte retrieval: Early vs. mid follicular endometrial priming. (Abstract Book 1) Hum Reprod 1996;11:2
Russell JB, Knezevich KM, Fabian KF, Dickson JA: Unstimulated immature oocyte retrieval: Early vs. mid follicular endometrial priming. Fertil Steril 1997;67:616-620
Barnes FL, Kausche A, Tiglias J, Wood C, Wilton L, Trounson A: Production of embryos from in vitro-matured primary human oocytes. Fertil Steril 1996;65:1151-1156
Trounson A, Bongso A, Szell A: Maturation of human and bovine primary oocytes in vitro for fertilization and embryo production. Singapore J Obstet Gynaecol 1996;27: 78-84
Chi HJ, Kim DH, Koo JJ, Chang SS: The suitability and efficiency of human follicular fluid as a protein supplement in human in vitro fertilization programs. Fertil Steril 1998;70: 871-877
Ménézo Y, Nicollet B, Herbaut N, Andre D: Freezing cocultured human blastocysts. Fertil Steril 1992;58:977-980
Son WY, Park SE, Lee KA, Lee WS, Ko JJ, Yoon TK, Cha KY: Effects of 1,2-propanediol and freezing-thawing on the in vitro developmental capacity of human immature oocytes. Fertil Steril 1996;66:995-999
Gardner DK, Lane M, Calderon I, Leeton J: Environment of the preimplantation human embryo in vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril 1996;65:349-353
Leese HJ, Lenton EA: Glucose and lactate in human follicular fluid: Concentrations and interrelationships. Hum Reprod 1990;5:915-919
Bavister BD: Culture of preimplantation embryos: Facts and artifacts. Hum Reprod Update 1995;1:91-148
Gardner DK, Lane M: Culture and selection of viable blastocysts: A feasible proposition for human IVF? Hum Reprod Update 1997;3:367-382
Yoon HG, Yoon SH, Heo YS, Yoon HJ, Lee SW, Lee YM, Park SP, Lee WD, Kim KA, Lim JH: High constant implantation rate and ongoing pregnancy rate obtained by control of embryo transfer date according to the quality and the number [abstract]. 54th meeting of the American Society for Reproductive Medicine, 1998
Smith SD, Mikkelsen A, Lindenberg S: Development of human oocytes matured in vitro for 28 or 36 hours. Fertil Steril 2000;73:541-544
Chian RC, Buckett WM, Tulandi T, Tan SL: Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000;15:165-170
Barnes FL, Zouves C, Rodriquez H, et al.: Clinical experience with in vitro maturation of human oocytes. In In Vitro Fertilization and Assisted Reproduction, V Gomel, PCK Leung (eds), Bologna, Monduzzi Editore, 1997, pp 139-144
Mikkelsen AL, Smith SD, Lindenberg S: In-vitro maturation of human oocytes from regularly menstruating women may be successful without follicle stimulating hormone priming. Hum Reprod 1999;14:1847-1851
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoon, HG., Yoon, SH., Son, WY. et al. Clinical Assisted Reproduction: Pregnancies Resulting from In Vitro Matured Oocytes Collected from Women with Regular Menstrual Cycle. J Assist Reprod Genet 18, 325–329 (2001). https://doi.org/10.1023/A:1016632621452
Issue Date:
DOI: https://doi.org/10.1023/A:1016632621452