Journal of Assisted Reproduction and Genetics

, Volume 19, Issue 8, pp 390–393 | Cite as

Successful Delivery Following Cryopreservation of Zygotes Produced by In Vitro Matured Oocytes Retrieved from a Woman with Polycystic Ovarian Syndrome-Like Disease: A Case Report

  • Koichi KyonoEmail author
  • Noritaka Fukunaga
  • Kosuke Haigo
  • Setsuyo Chiba
  • Chisako Ohira
  • Yukiko Nakajo
  • Yasuhisa Araki


Purpose: To estimate frozen zygotes, which developed from in vitro matured oocytes retrieved from polycystic ovarian syndrome-like disease.

Methods: Oocyte retrieval was performed on Day 15 following withdrawal bleeding. The oocytes were incubated for 24 h in TCM-199 maturation medium supplemented with follicle fluid, E2, FSH, and hCG.

Results: A total of 12 immature oocytes were collected. Seven of the 12 oocytes (58.3%) developed to the metaphase-II stage, and subsequently, all seven fertilized oocytes were frozen at the pronuclear stage. The remaining five oocytes failed to develop to the metaphase-II stage after an additional 24 h of incubation. Three of seven cryopreserved oocytes were thawed and developed to 2–8-cell cleaved stage embryos. The first pregnancy failed. However, the second frozen–thawed embryo transfer resulted in the delivery of healthy twins.

Conclusions: Successful delivery using frozen zygotes from an anovulatory woman with polycystic ovarian syndrome-like disease.

Cryopreservation ICSI mature oocyte PCOS 


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Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Koichi Kyono
    • 1
    Email author
  • Noritaka Fukunaga
    • 1
  • Kosuke Haigo
    • 1
  • Setsuyo Chiba
    • 1
  • Chisako Ohira
    • 1
  • Yukiko Nakajo
    • 1
  • Yasuhisa Araki
    • 2
  1. 1.Kyono Reproduction Research Center and Lady's Clinic KyonoFurukawa, MiyagiJapan
  2. 2.The Institute for ARMTIshii, Fujimi, Setagun, GunmaJapan

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