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Isolation and Expression Analysis of the Human RNH2 Gene Encoding Ribonuclease Inhibitor 2

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Abstract

Ribonuclease inhibitor 1 (RI-1) is a cytoplasmic protein that inhibits a variety of pancreatic-type mammalian Rnases by forming a very tight, reversible 1:1 complex. Recently a novel gene encoding ribonuclease inhibitor 2, Rnh2 has been isolated in mouse. The expression pattern of the mouse Rnh2 is specific to testis, especially in the spermatogonia. Then it has been suggested that the mouse Rnh2 gene may play critical roles in mouse spermatogenesis. In this study, we isolated the human RNH2 cDNA and analyzed its expression pattern. The human RNH2 is also expressed limited to testis, and then it is suggested that the human Rnh2 gene may also play critical roles in human spermatogenesis.

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REFERENCES

  1. Lee FS, Vallee BL: Structure and action of mammalian ribonuclease (angiogenin) inhibitor. Prog Nucl Acid Res 1993;44:1-30

    Google Scholar 

  2. Hofsteenge J: ‘Holy’ proteins I: Ribonuclease inhibitor. Curr Opin Struct Biol 1994;4:807-809

    Google Scholar 

  3. Hofsteenge J, Kieffer B, Matthies R, Hemmings BA, Stone SR: Amino acid sequence of the ribonuclease inhibitor from porcine liver reveals the presence of leucine-rich repeats. Biochemistry 1988;27:8537-8544

    Google Scholar 

  4. Lee FS, Fox EA, Zhou HM, Strydom DJ, Vallee BL: Primary structure of human placental ribonuclease inhibitor. Biochemistry 1988;27:8545-8553

    Google Scholar 

  5. Schneider R, Schneider-Scherzer E, Thurnher M, Auer B, Schweiger M: The primary structure of human ribonuclease/ angiogenin inhibitor (RAI) discloses a novel highly diversified protein superfamily with a common repetitive module. EMBO J 1988;7:4151-4156

    Google Scholar 

  6. Neumann U, Hofsteenge J, Arkema AH, Dijkstra BW: Crystalization of porcine liver ribonuclease inhibitor, a member of the family of proteins containing leucine-rich repeats. J Mol Biol 1993;231:505-508

    Google Scholar 

  7. Wang PJ, McCarrey JR, Yang F, Page DC: An abundance of X-linked genes expressed in spermatogonia. Nat Genet 2001;27:422-426

    Google Scholar 

  8. Andrade MA, Perez-Iratxeta C, Ponting CP: Protein repeats: Structure, function, and evolution. J Struct Biol 2001;134:117-131

    Google Scholar 

  9. Kobe B, Deisenhofer J: A structure basis of the interactions between leucine-rich repeats and protein ligands. Nature 1995;374:183-186

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Mammalian Genes and Development, National Institute of Child Health, and Human Development, NIH, Building 6B Room 2B211, 9000 Rockville Pike, Bethesda, Maryland, 20892

    Toshinobu Miyamoto & Shiga Hasuike

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  1. Toshinobu Miyamoto
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  2. Shiga Hasuike
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Miyamoto, T., Hasuike, S. Isolation and Expression Analysis of the Human RNH2 Gene Encoding Ribonuclease Inhibitor 2. J Assist Reprod Genet 19, 394–397 (2002). https://doi.org/10.1023/A:1016302706156

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  • DOI: https://doi.org/10.1023/A:1016302706156

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