Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation
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Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. Percentages of anti-smooth muscle α-actin labelled cells were assessed at early and late phase by flow cytofluorometric analysis to control the effect of heparin. At 100 μg ml-1, no effect on α-actin labelled cell growth has been detected. This result contrasted with the inhibition of monolayer cell cultures. At 500 μg ml-1, the proliferation of smooth muscle cells was reduced. This explant system should be useful for testing drugs susceptible to interfere with restenosis.
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- Abramovitch R, Neeman M, Reich R, Stein I, Keshet E, Abraham J, Solomon A and Marikovsky M (1998) Intercellular communication between vascular smooth muscle and endothelial cells mediated by heparin-binding epidermal growth factor-like growth factor and vascular endothelial growth factor. FEBS Lett 425:441–447.PubMedCrossRefGoogle Scholar
- Christ G, Wojta J and Binder BR (1992) Distribution of smooth muscle cells derived urokinase in cocultures with endothelial cells.Fibrinolysis 6:139–143.Google Scholar
- Fager G, Camejo G and Bondjers G (1992) Heparin-like glycosaminoglycans influence growth and phenotype of human arterial smooth muscle cells in vitro. I. Evidence for reversible binding and inactivation of the PDGF by heparin. In Vitro Cell Dev Biol 28:168–175.Google Scholar
- Gross DR (1985) Animal Models in Cardiovascular Research. Boston, Martinus Nijhoff, pp.537–547.Google Scholar
- Hardhammer PA, van Beusekom HM, Emanuelsson SH, Hofma SH, Albertssson PA, Verdouw PD, Boersma E, Serruys PW and van der Giessen WJ (1996) Reduction in thrombotic events with heparin-coated Palmaz-Schatz stents in normal porcine coronary arteries.Ciculation93:423–430.Google Scholar
- Kinard F, Sergent-Engelen T, Trouet A, Remacle C and Schneider YJ (1997) Compartmentalized coculture of porcine arterial endothelial and smooth muscle cells on a microporous membrane. In Vitro Cell Dev Biol 33:92–103.Google Scholar
- Oberhoff M, Novak S, Herdeg C, Baumbach A, Kranzhofer AGoogle Scholar
- Saunders KB and D'Amore PA (1992) An in vitromodel for cell- cell interactions. In Vitro Cell Dev Biol28:521–528.Google Scholar