, Volume 37, Issue 1, pp 13–22 | Cite as

Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation

  • M. DufresneEmail author
  • R. Warocquier-Clérout


Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. Percentages of anti-smooth muscle α-actin labelled cells were assessed at early and late phase by flow cytofluorometric analysis to control the effect of heparin. At 100 μg ml-1, no effect on α-actin labelled cell growth has been detected. This result contrasted with the inhibition of monolayer cell cultures. At 500 μg ml-1, the proliferation of smooth muscle cells was reduced. This explant system should be useful for testing drugs susceptible to interfere with restenosis.

coronary artery explant culture endothelial cells heparin proliferation smooth muscle cells 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  1. 1.Laboratoire de Biologie Cellulaire ExpérimentaleUMR 6600, Université de Technologie de CompiègneCompiègneFrance
  2. 2.Laboratoire de Biologie Cellulaire ExpérimentaleUMR 6600, Université de Technologie de CompiègneCompiègneFrance

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