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Cytotechnology

, Volume 37, Issue 1, pp 49–53 | Cite as

Microsupport with two-dimensional geometry (2D-MS). In situ determination of the growth kinetics of anchorage-dependent cells by Laser Diffraction Particle Sizing (LDPS)

  • N. Kenda-Ropson
  • D. Mention
  • V. Motte
  • M. Genlain
  • A. O. A. MillerEmail author
Article

Abstract

The procedure used to establish in situ (without cell trypsinization) the growth kinetics characteristics of anchorage-dependent cells propagated on microcarriers by Aperture Impedance Pulse Spectroscopy can be replaced by a novel method based on the time-dependent shifts of the size distribution histograms of cell-laden microcarriers. This we have named Laser Diffraction Particle Sizing.

anchorage-dependent cells biomass microcarrier probe 

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References

  1. Degouys V, Menozzi FD, Dubois DA and Miller AOA (1996a) Electronic sizing of microbeads covered with cells: In situbiomass evaluation by Aperture Impedance Pulse Spectroscopy in Cell and Tissue Culture: Laboratory Procedures. In: A Doyle, JB Griffiths and DG Newell (eds) (28C:3.1), John Wiley & Sons.Google Scholar
  2. Degouys V, Menozzi FD, Harfield J, Fabry L and Miller AOA (1996b) Electronic Estimation of the Biomass in Animal Cell Cultures.Chemistry Today 14:33–36.Google Scholar
  3. Degouys V, Harfield J, Fabry L, Menozzi FD and Miller AOA (1996c) Fast in situdetermination of the Biomass of anchoragedependent cells.Cytotechnol 19:107–110.CrossRefGoogle Scholar
  4. Gatot C, Trampler F, Wanderpepen M-P, Harfield J, Oudshoorn A, Johansson A, Nielsen V and Miller AOA (1998) Use of a new microcarrier with two dimensional geometry for the culture of anchorage-dependent cells in sonoperfused, continuously stirred tank reactors. In: O-W Merten, P Perrin and B Griffiths (eds), New Developments and New Applications in Animal Cell Technology - Proceedings of the 15th ESACT Meeting (p.377) Kluwer Academic Publishers, Dordrecht.Google Scholar
  5. Miller SJO, Henrotte M and Miller AOA (1986) Growth of Animal Cells on Microbeads 1.In situEstimation of numbers Biotechnol Bioeng 28:1466–1473.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • N. Kenda-Ropson
    • 1
  • D. Mention
    • 1
  • V. Motte
    • 1
  • M. Genlain
    • 1
  • A. O. A. Miller
    • 2
    Email author
  1. 1.4C Biotech s.a.SeneffeBelgium
  2. 2.4C Biotech s.a.SeneffeBelgium

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