Skip to main content
Log in

Microsupport with two-dimensional geometry (2D-MS). In situ determination of the growth kinetics of anchorage-dependent cells by Laser Diffraction Particle Sizing (LDPS)

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The procedure used to establish in situ (without cell trypsinization) the growth kinetics characteristics of anchorage-dependent cells propagated on microcarriers by Aperture Impedance Pulse Spectroscopy can be replaced by a novel method based on the time-dependent shifts of the size distribution histograms of cell-laden microcarriers. This we have named Laser Diffraction Particle Sizing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Degouys V, Menozzi FD, Dubois DA and Miller AOA (1996a) Electronic sizing of microbeads covered with cells: In situbiomass evaluation by Aperture Impedance Pulse Spectroscopy in Cell and Tissue Culture: Laboratory Procedures. In: A Doyle, JB Griffiths and DG Newell (eds) (28C:3.1), John Wiley & Sons.

  • Degouys V, Menozzi FD, Harfield J, Fabry L and Miller AOA (1996b) Electronic Estimation of the Biomass in Animal Cell Cultures.Chemistry Today 14:33–36.

    CAS  Google Scholar 

  • Degouys V, Harfield J, Fabry L, Menozzi FD and Miller AOA (1996c) Fast in situdetermination of the Biomass of anchoragedependent cells.Cytotechnol 19:107–110.

    Article  Google Scholar 

  • Gatot C, Trampler F, Wanderpepen M-P, Harfield J, Oudshoorn A, Johansson A, Nielsen V and Miller AOA (1998) Use of a new microcarrier with two dimensional geometry for the culture of anchorage-dependent cells in sonoperfused, continuously stirred tank reactors. In: O-W Merten, P Perrin and B Griffiths (eds), New Developments and New Applications in Animal Cell Technology - Proceedings of the 15th ESACT Meeting (p.377) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Miller SJO, Henrotte M and Miller AOA (1986) Growth of Animal Cells on Microbeads 1.In situEstimation of numbers Biotechnol Bioeng 28:1466–1473.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. O. A. Miller.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kenda-Ropson, N., Mention, D., Motte, V. et al. Microsupport with two-dimensional geometry (2D-MS). In situ determination of the growth kinetics of anchorage-dependent cells by Laser Diffraction Particle Sizing (LDPS). Cytotechnology 37, 49–53 (2001). https://doi.org/10.1023/A:1016156107753

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016156107753

Navigation