Abstract
Purpose : Testicular biopsy specimens contain large amounts of debris that makes sperm pick-up for ICSI more difficult than with epididymal aspirates. We sought to develop improved processing techniques for testicular sperm extraction (TESE).
Methods : Retrievals were with azoospermic male partner scheduled to undergo percutaneous epididymal sperm aspiration (PESA) and TESE. The study group consisted of 9 retrievals with a new TESE technique (TESE-N). The control group was 21 retrievals with PESA and 3 retrievals with a previous TESE technique (TESE-P).
Results : TESE-N eliminated almost all debris, which made ICSI sperm pick-up more rapid. TESE-N, PESA, and TESE-P fertilization (77, 75, and 72%) and ongoing/delivered pregnancy rates per retrieval (67, 76, and 67%) were similar.
Conclusions : Our new technique provides for easy removal of debris from TESE specimens and fertilization and pregnancy rates equal to epididymal sperm's. Eliminating debris from TESE specimens allows for rapid sperm pick-up for ICSI, making the procedure more efficient for embryology staff.
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REFERENCES
Craft IL, Khalifa Y, Boulos A, Pelekanos M, Foster C, Tsirigotis M: Factors influencing the outcome of in vitro fertilization with percutaneous aspirated spermatozoa and intracytoplasmic sperm injection in azoospermic men.HumReprod 1995;10:1791–1794
Liu DY, Tsai Y, Katz E: Outcome of in-vitro culture of fresh and frozen thawed human testicular spermatozoa. Hum Reprod 1997;12:1667–1672
Nagy Z, Liu J, Cecile J, Silber S, Devroey P, Van Steirteghem A: Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil Steril 1995;63:808–815
American Society for Reproductive Medicine, Practice Committee Report: New techniques for sperm acquisition in obstructive azoospermia. Technical Bulletin, Aug 1999
Balaban B, Urman B, Sertac A, Alatas C, Aksoy S, Mercan R, Nuhoglu A: In-vitro culture of spermatozoa induces motility and increases implantation and pregnancy rates after testicular sperm extraction and intracytoplasmic sperm injection. Hum Reprod 1999;14:2808–2811
Nagy A, Verheyen G, Tournaye H, Devroey P, Van Steirteghem AC: An improved treatment procedure for testicular biopsy specimens offers more efficient sperm recovery: Case series. Fertil Steril 1997;68:376–379
Sheynkin Y, Schlegel PN: Sperm retrieval for assisted reproductive technologies. Contemp Urol 1997;Sept:289–298
Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, Van Steirteghem A, Silber S: Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in nonobstructive azoospermia. Hum Reprod 1995;10:1457–1460
Rosenlund B, Sjoblom P, Dimitrakopoulos, Hillensjo T: Epididymal and testicular sperm for intracytoplasmic sperm injection in the treatment of obstructive azoospermia. Acta Obstet Gynecol Scand 1997;75:135–139
Silber SJ, Van Steirteghem AC, Liu J, Nagy Z, Tournaye H, Devroey P: High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy. Hum Reprod 1995;10:148–152
Strehler E, Baccetti B, Sterzik K, Capitani S, Collodel G, de Santo M, Gambera L, Piomboni P: Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa. Hum Reprod 1998;13:120–123
Jean M, Mirallie S, Boudineau M, Tatin C, Barriere P: Intracytoplasmic sperm injection with polyvinylpyrrolidone. Fertil Steril 2001;76:419–420
Aitken RJ, Gordon E, Harkiss D: Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod 1998;59:1037–1046
Iwasaki A, Gagnon C: Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992;57:409–416
Damario MA, Hammitt DG, Galanits TM, Session DR, Dumesic DA: Pronuclear stage cryopreservation after intracytoplasmic sperm injection and conventional IVF: Implications for timing of the freeze. Fertil Steril 1999;72:1049–1054
Damario MA, Hammitt DG, Session DR, Dumesic DA: Embryo cryopreservation at the pronuclear stage and efficient embryo use optimizes the chance for a liveborn infant from a single oocyte retrieval. Fertil Steril 2000;73:767–773
Tournaye H, Liu J, Nagy PZ, Camus M, Goossens A, Silber S, Van Steirteghem AC, Devroey P: Correlation between testicular histology and outcome after intracytoplasmic sperm injection using testicular spermatozoa. Hum Reprod 1996;11:127–132
Zhu J, Tsirigotis M, Pelekanos M: In-vitro maturation of human testicular spermatozoa. Hum Reprod 1996;11:231–232
Nagy Z, Liu J, Cecile J, Joris H, Verheyen G, Tournaye H, Camus M, Derde MP, Devroey P, Van Steirteghem A: The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. HumReprod 1995;10:1123–1129
Tasdemir I, Tasdemir M, Ravukcuoglu S: Effect of pentoxifylline on immotile testicular spermatozoa. J Assist Reprod Genet 1998;15:90–92
Tomlinson MJ, Moffatt O, Manicardi GC, Bizzaro D, Afnan M, Sakkas D: Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: Implications for assisted conception. Hum Reprod 2001;16:2160–2165
Ollero M, Gil-Guzman E, Lopez MC, Sharma RD, Agarwal A, Larson K, Evenson D, Thomas AJ, Alvarez JG: Characterization of subsets of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility. Hum Reprod 2001;16:1912–1921
Kovanci E, Kovacs T, Moretti E, Vigue L, Bray-Ward P, Ward D, Huszar G: FISH assessment of aneuploidy frequencies in mature and immature human spermatozoa classified by the absence of presence of cytoplasmic retention. Hum Reprod 2001;16:1209–1217
Rubio C, Gil-Salom M, Simon C, Vidal F, Rodrigo L, Minguez Y, Remohi J, Pellicer A: Incidence of sperm chromosomal abnormalities in a risk population: Relationship with sperm quality and ICSI outcome and immature human spermatozoa classified by the absence of presence of cytoplasmic retention.Hum Reprod 2001;16:2084–2092
Egozcue S, Blanco J, Vendrell JM, Garcia F, Veiga A, Aran B, Barri PN, Vidal F, Egozcue J: Human male infertility: Chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update 2000;6:93–105
Emiliani S, Van den Bergh M, Vannin AS, Biramane J, Verdoodt M, Englert Y: Evidence of reduced single-stranded testicular sperm DNA from obstructive azoospermic men after 3 days of in-vitro culture. Hum Reprod 2001;16: 1200–1203
Allan JA, Cotman AS: A new method for freezing testicular biopsy sperm: Three pregnancies with sperm extracted from cryopreserved section of seminiferous tubule. Fertil Steril 1997;68:741–744
Verheyen G, De Croo I, Tournaye H, Pletinex I, Devroey P, Van Steirteghem AC: Comparison of four mechanical methods to retrieve spermatozoa from testicular tissue. Hum Reprod 1995;10:2956–2959
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Hammitt, D.G., Ferrigni, R.G., Sattler, C.A. et al. Development of a New and Efficient Laboratory Method for Processing Testicular Sperm. J Assist Reprod Genet 19, 335–342 (2002). https://doi.org/10.1023/A:1016010709945
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DOI: https://doi.org/10.1023/A:1016010709945