Abstract
A 1397-bp fragment corresponding to the rpoC2 chloroplast RNA polymerase gene was obtained by direct rye DNA amplification. Two rye species, Secale montanum Guss. and S. cereale L., did not practically differ in the structure of this DNA fragment (the nucleotide sequences were 99% identical). The corresponding nucleotide sequences in rye and wheat (Triticum aestivum L., Genbank accession no. AB027572) were 97–98% similar. The extent of the homology of various stretches of the rpoC2 rye gene with the corresponding sequences in maize and rice was 81–95%, whereas the deduced amino acid sequences of rpoC2 in rye, wheat, maize, and rice were considerably identical (96–97% of homology). The rye fragment of the rpoC2 gene differed from the corresponding sequences in three other grass species primarily by a short (49 bp) insert into the region of numerous short repeats corresponding to nucleotides 15750/15751, 28728/28729, and 27472/27473 in wheat, maize, and rice, respectively.
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REFERENCES
Maliga, P., Two Plastid RNA Polymerases of Higher Plants: An Evolving Story, Trends Plant Sci., 1998, vol. 3, pp. 4-6.
Hess, W.R. and Börner, T., Organellar RNA Polymerases of Higher Plants, Int. Rev. Cytol., 1999, vol. 190, pp. 1-59.
Igloi, G.L., Meinke, A., Dory, I., and Kossel, H., Nucleotide Sequence of the Maize Chloroplast rpo B/C1/C2 Operon: Comparison between the Derived Protein Primary Structures from Various Organisms with Respect to Functional Domains, Mol. Gen. Genet., 1990, vol. 221, pp. 379-394.
Shimada, H. and Sugiura, M., Fine Structural Features of the Chloroplast Genome: Comparison of the Sequenced Chloroplast Genomes, Nucleic Acids Res., 1991, vol. 19, pp. 983-995.
Cummings, M.P., King, L.M., and Kellogg, E.A., Slipped-Strand Mispairing in a Plastid Gene: rpoC2 in Grasses (Poaceae), Mol. Biol. Evol., 1994, vol. 11, pp. 1-8.
Maier, R.M., Neckermann, K., Igloi, G.L., and Kossel, H., Complete Sequence of the Maize Chloroplast Genome: Gene Content, Hotspots of Divergence and Fine Tuning of Genetic Information by Transcript Editing, J. Mol. Biol., 1995, vol. 251, pp. 614-628.
Chen, Z., Schertz, K.F., Mullet, J.E., DuBell, A., and Hart, G.E., Characterization and Expression of rpoC2 in CMS and Fertile Lines of Sorghum, Plant Mol. Biol., 1995, vol. 28, pp. 799-809.
Katayama, H. and Ogihara, Y., Phylogenetic Affinities of the Grasses to Other Monocots as Revealed by Molecular Analysis of Chloroplast DNA, Curr. Genet., 1996, vol. 29, pp. 572-581.
Ohnishi, Y., Tajiri, H., Matsuoka, Y., and Tsunewaki, K., Molecular Analysis of a 21.1-kb Fragment of Wheat Chloroplast DNA Bearing RNA Polymerase Subunit (rpo) Genes, Genome, 1999, vol. 42, pp. 1042-1049.
Hess, W.R., Prombona, A., Fieder, B., Subramanian, A.R., Börner, T., Chloroplast rps15 and the rpoB/C1/C2 Gene Cluster Are Strongly Transcribed in Ribosome-Deficient Plastids: Evidence for a Functioning Non-Chloroplast-Encoded RNA Polymerase, EMBO J., 1993, vol. 12, pp. 563-571.
Akopian, T.A., Vasil'ev, S.A., Ermishev, V.Yu., Zabrodina, M.V., Karyagina, A.S., Naroditsky, B.S., and Khavkin, E.E., Conserved Fragments of the MADS-Box Genes in Perennial and Annual Rye, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 100-107 (Russ. J. Plant Physiol., Engl. Transl.).
Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J., Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Searchprograms, Nucleic Acids Res., 1997, vol. 25, pp. 3389-3402.
Shimada, H. and Sugiura, M., Pseudogenes and Short Repeated Sequences in the Rice Chloroplast Genome, Curr. Genet., 1989, vol. 16, pp. 293-301.
Ogihara, Y., Terachi, T., and Sasakuma, T., Molecular Analysis of the Hot Spot Region Related to Length Mutations in Wheat Chloroplast DNAs: 1. Nucleotide Divergence of Genes and Intergenic Spacer Regions Located in the Hot Spot Region, Genetics, 1991, vol. 129, pp. 873-884.
Clegg, M.T., Gaut, B.S., Learn, G.H., Jr, and Morton, B.R., Rates and Patterns of Chloroplast DNA Evolution, Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 6795-6801.
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Ermishev, V.Y., Naroditsky, B.S. & Khavkin, E.E. A Fragment of the rpoC2 Chloroplast RNA Polymerase Gene in Perennial and Annual Rye. Russian Journal of Plant Physiology 49, 387–392 (2002). https://doi.org/10.1023/A:1015553321314
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DOI: https://doi.org/10.1023/A:1015553321314