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Noncontact, Laser-Mediated Extraction of Polar Bodies for Prefertilization Genetic Diagnosis

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Abstract

Purpose: We tested an entirely noncontact polar body-extraction method using an ultraviolet laser beam for laser zona drilling and a near infrared laser beam for polar body (PB) trapping and extraction. Methods: A hole was drilled into the zona pellucida of an oocyte. Then, the PB was trapped with optical tweezers and dragged through the drilled hole. Results: Bovine first PBs could be extracted in 49 out of 63 oocytes (78%) using this method. In human oocytes, PB extraction was successfully demonstrated, which however was more time consuming. A number of extracted PBs were dried on a special membrane, circumcised with the laser microbeam, and successfully catapulted into the lid of a microfuge tube (laser pressure catapulting). Conclusions: This solely laser-mediated extraction method allows convenient procurement of PBs without the danger of contamination and is a promising approach that might replace standard micromanipulation methods in the future.

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Correspondence to A. Clement-Sengewald.

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Clement-Sengewald, A., Buchholz, T., Schütze, K. et al. Noncontact, Laser-Mediated Extraction of Polar Bodies for Prefertilization Genetic Diagnosis. J Assist Reprod Genet 19, 183–194 (2002). https://doi.org/10.1023/A:1014894029099

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  • DOI: https://doi.org/10.1023/A:1014894029099

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