Abstract
Purpose : This study was conducted to determine the gene expression of insulin-like growth factors (IGFs), their receptors, and binding proteins (IGFBPs) in human luteinized granulosa cells collected during ovum retrieval.
Methods : cDNA probes were used to analyze the gene expression of IGF-I, IGF-II, IGF type 1 receptor (IGFR-1), IGF type 2 receptor (IGFR-2), and IGFBP-1 to -6 in luteinized granulosa cells.
Results : The levels of IGFBP-5 transcripts of the luteinized granulosa cells increased with increasing follicular fluid (FF) volume when follicles were grouped into 3 volume catagories of ≤1.5 mL; >1.5 mL but ≤4.5 mL; and >4.5 mL (0.28 ± 0.64 vs. 0.41 ± 0.76 vs. 0.46 ± 0.92, p = 0.037).
Conclusions : The increased levels of IGFBP-5 transcripts in luteinized granulosa cells found with increased follicular fluid volume after COH may favor a shift toward diminished action of IGFs in larger follicles immediately prior to ovulation.
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Chang, S.Y., Tsai, MY., Huang, FJ. et al. Expression of Insulin-Like Growth Factor (IGF), IGF Receptor, and IGF-Binding Protein Messenger Ribonucleic Acids in Luteinized Granulosa Cells from Different Size Follicles After Controlled Ovarian Hyperstimulation. J Assist Reprod Genet 19, 121–126 (2002). https://doi.org/10.1023/A:1014732704218
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DOI: https://doi.org/10.1023/A:1014732704218