Abstract
Purpose : Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium.
Materials and Methods : Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF–liposome (Group 1) and luciferase–liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0–4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection.
Results : LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1–4 were 0.414, 1.096, 1.162, and 0.782. In Group 2, LIF/GADPH on Days 1–4 were 0.24, 0.22, 0.35, and 0.32.
Conclusions : Mouse endometrium could be effectively transfected with liposome–DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.
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Hsieh, YY., Lin, CS., Sun, YL. et al. In Vivo Gene Transfer of Leukemia Inhibitory Factor (LIF) into Mouse Endometrium. J Assist Reprod Genet 19, 79–83 (2002). https://doi.org/10.1023/A:1014495614792
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DOI: https://doi.org/10.1023/A:1014495614792