Abstract
Purpose: Evaluation of vascular endothelial growth factor (VEGF) concentrations of follicular fluid (FF) in accordance with an ovarian stimulation protocol and ovarian response.
Methods: The subjects of this study were 38 patients undergoing IVF-ET in our hospital. They were divided into three groups according to ovarian stimulation protocols; Group 1: hMG cycles (n = 19), Group 2: clomiphene citrate cycles (n = 10), Group 3: natural cycles (n = 9). They reclassified into three groups according to the number of oocytes harvested. VEGF concentration was measured in FF, employing ELISAs.
Results: Group 1 shows lower VEGF concentrations in FF than Group 2 or Group 3. Excluding high responders from Group 1, no difference was found among these three groups. As for the reclassified groups, group of highest number of oocytes havested showed lowest VEGF concentrations in FF.
Conclusions: VEGF concentrations in FF might negatively correlate with the number of follicles, irrespective of the ovulation induction protocol.
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REFERENCES
Findlay JK: Angiogenesis in reproductive tissue. J Endocr 1986;111:357-366
Ferrera N, Houck K, Jakeman L, Leung DW: Molecular and biological properties of the VEGF family of proteins. Endocr Rev 1992;1:18-32
Lee A, Burry KA, Christenson LK, Patton PE, Stouffer RL: Vascular endothelial growth factor levels in serum and follicular fluid of patients undergoing in vitro fertilization. Fertil Steril 1997;68:305-311
Abramov Y, Elshalal U, Shenker JG: Severe OHSS, An “epidemic of OHSS”: A price we have to pay. Hum Reprod 1999;14:2181-2185
McClure N, Healy DL, Roger PA, Sullivan J, Beaton L, Haning RV Jr, Connolly DT, Robertson DM: Vascular endothelial growth factor as capillary permeability agent in ovarian hyperstimulation syndrome. Lancet 1994;344:235-236
Rizk B, Aboulghar M, Smitz J, Ron-El R: The role of vascular endothelial growth factor and interleukins in the pathogenesis of severe ovarian hyperstimulation syndrome. Hum Reprod Update 1997;3:255-266
Abramov Y, Barak V, Nisman B, Shenker JG: Vascular endothelial growth factor plasma levels correlate to the clinical picture in severe ovarian hyperstimulation syndrome. Fertil Steril 1997;67:261-265
Artini PG, Fasciani A, Monti M, Luisi S, D'Ambrogio G, Genazzani AR: Changes in vascular endothelial growth factor levels and the risk of ovarian hyperstimulation syndrome in women enrolled in an in vitro fertilization program. Fertil Steril 1998;70:560-564
Agrawal R, Tan SL, Wild S, Sladkevicius P, Engmann L, Payne N, Bekir J, Campbell S, Conway G, Jacobs H: Serum vascular endothelial growth factor concentrations in in vitro fertilization cycles predict the risk of ovarian hyperstimulation syndrome. Fertil Steril 1999;71:287-293
Chen CD, Chen HF, Lu HF, Chen SU, Ho HN, Yang YS: Value of serum and follicular fluid cytokine profile in the prediction of moderate to severe ovarian hyperstimulation syndrome. Hum Reprod 2000;15:1037-1042
Geva E, Amit A, Lessing JB, Lerner-Geva L, Daniel Y, Yovel I, Azem F, Barak V: Follicular fluid levels of vascular endothelial growth factor. Are they predictive markers for ovarian hyperstimulation syndrome? J Reprod Med 1999;44:91-96
Lee A, Christenson LK, Patton PE, Burry KA, Stouffer RL: Vascular endothelial growth factor production by human luteinized granulosa cells in vitro. Hum Reprod 1997;12:2756-2761
Anasti JN, Kalantaridou SN, Kimzey LM, George M, Nelson LM: Human follicle fluid vascular endotherial growth factor concentrations are correlated with luteinization in spontaneously developing follicles. 1998;13:1144-1147
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Tokuyama, O., Nakamura, Y., Muso, A. et al. Vascular Endothelial Growth Factor Concentrations in Follicular Fluid Obtained from IVF-ET Patients: A Comparison of hMG, Clomiphene Citrate, and Natural Cycle. J Assist Reprod Genet 19, 19–23 (2002). https://doi.org/10.1023/A:1014006605859
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DOI: https://doi.org/10.1023/A:1014006605859