Abstract
Purpose: To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, P1 (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation.
Methods: Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or P1 supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium.
Results and Conclusion: In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.
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Ben-Yosef, D., Yovel, I., Schwartz, T. et al. Increasing Synthetic Serum Substitute (SSS) Concentrations in P1 Glucose/Phosphate-Free Medium Improves Implantation Rate: A Comparative Study. J Assist Reprod Genet 18, 588–592 (2001). https://doi.org/10.1023/A:1013156804589
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DOI: https://doi.org/10.1023/A:1013156804589