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Efficiency of Using Frozen-Thawed Testicular Sperm for Multiple Intracytoplasmic Sperm Injections

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Abstract

Purpose: To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI).

Methods: Sperm collected from the testes of 28 azoospermic patients by an open testicular biopsy technique was used for initial ICSI or cryopreserved.

Results: Fresh-sperm ICSI treatment (28 cycles) resulted in a 58.1% fertilization rate and a 32.1% clinical pregnancy rate per embryo transfer, while frozen-thawed sperm (24 subsequent cycles) had rates of 54.5 and 29.2%, respectively. The PR was lower using frozen-thawed sperm from nonobstructive azoospermia patients (9.1%) than from obstructive azoospermia patients (46.2%). PR declined to 0% upon the fourth ICSI attempt.

Conclusions: Fertilization, embryo cleavage, and pregnancy rates were unaffected by fresh or frozen-thawed sperm use. A 57.1% cumulative clinical PR was achieved using the latter. The PR was significantly lower using frozen-thawed sperm from nonobstructive azoospermia patients than from obstructive azoospermia patients.

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Authors and Affiliations

  1. Ladies Clinic Kyono, 3-8-6, Oomiya, Furukawa, Miyagi, 989-6221, Japan

    Noritaka Fukunaga, Kosuke Haigo & Koichi Kyono

  2. The Institute for ARMT, 2-39-3, Kamikoide, Maebashi, Gunma, 371-0037, Japan

    Yasuhisa Araki

Authors
  1. Noritaka Fukunaga
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  2. Kosuke Haigo
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  3. Koichi Kyono
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  4. Yasuhisa Araki
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Fukunaga, N., Haigo, K., Kyono, K. et al. Efficiency of Using Frozen-Thawed Testicular Sperm for Multiple Intracytoplasmic Sperm Injections. J Assist Reprod Genet 18, 634–637 (2001). https://doi.org/10.1023/A:1013107132110

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  • DOI: https://doi.org/10.1023/A:1013107132110

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