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Study of the In Vitro Maturation of Mouse Oocytes Induced by Microinjection of Maturation Promoting Factor (MPF)

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Abstract

Purpose: Maturation promoting factor (MPF) acts at theresumption of meiosis and nonspecifically throughout theanimal species. There exists a considerable body of literatureon MPF, but little work has been done to study the inductionof maturation of mammalian oocytes by microinjection ofextracted MPF.

Methods: Immature (GV-stage) mouse oocytes weremicroinjected MPF extracted from matured Xenopus eggs in thepresense of dbcAMP.

Results: The rate of germinal vesicle breakdown (GVBD)induced at 24 hr after MPF injection was significantly higher(90.5%) than that of the control (2.2%), which was injectedwith HTF medium containing dbcAMP (P < 0.0001). Therate of extrusion of the first polar body at 24 hr after MPFinjection was significantly higher (84.1%) than that of thesame control (1.1%) (P < 0.0001).

Conclusions: From these results, it is concluded that thematuration of mammalian oocytes can be induced by themicroinjection of MPF extracted from other species.

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REFERENCES

  1. Masui Y, Markert CL: Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes. J Exp Zool 1971;177:129-146

    Google Scholar 

  2. Kishimoto T: Regulation of metaphase by a maturation-promoting factor. Dev Growth Different 1988:30:105-115

    Google Scholar 

  3. Hashimoto N, Kishimoto T: Regulation of miotic metaphse by a cytoplasmic maturation-promoting factor during mouse oocyte maturation. Dev Biol 1988; 126:242-252

    Google Scholar 

  4. Sorensen RA, Cyert MS, Pedersen RA: Active maturation-promoting factor is present in mature mouse oocytes. J Cell Biol 1985;100:1637-1640

    Google Scholar 

  5. Miake-Lye R, Newport J, Kirschner M: Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis. J Cell Biol 1983;97:81-91

    Google Scholar 

  6. Balakier H, Czolowska R: Cytoplasmic control of nuclear maturation in mouse oocytes. Exp Cell Res 1977;110:466-469

    Google Scholar 

  7. Fulka J Jr: Nuclear maturation in pig and rabbit oocytes after interspecific fusion. Exp Cell Res 1983;146:212-218

    Google Scholar 

  8. Prochazka R, Motlic J, Fulka J: Activity of maturation promoting factor in pig oocytes after microinjection and serial transfer of maturing cytoplasm. Cell Different Dev 1989;27:175-182

    Google Scholar 

  9. Kishimoto T, Yamazaki K, Kato Y, et al.: Induction of starfish oocyte maturation by maturation-prommoting factor of mouse and surf clam oocytes. J Exp Zool 1984;231:293-295

    Google Scholar 

  10. Sunkara PS, Wright DA, Rao PN: Mitotic factors from mammalian cells induce germinal vesicle breakdown and chromosome condensation in amphibian oocytes. Proc Natl Acad Sci USA 1979;76:2799-2802

    Google Scholar 

  11. Lohka MJ, Hayes MK, Maller JL: Purification of maturation-promoting factor, an intracellular regulator of early mitotic events. Proc Natl Acad Sci USA 1988;85:3009-3013

    Google Scholar 

  12. Yamashita S, Maller JL: Identification of an activator required for elevation of maturation-promoting factor(MPF) activity by ?-s-ATP. J Cell Biol 1990;110:1583-1588

    Google Scholar 

  13. Kimura Y, Yanagimachi R: Intracytoplasmic sperm injection in the mouse. Biol Reprod 1995; 52:709-720

    Google Scholar 

  14. Van deWiel DFM, Bar-Ami S, Tsafriri A, et al.: Oocyte maturation inhibitor, inhibin and steroid concentrations in porcine follicular fluid at various stages of the estrous cycle. J Reprod Fertil 1983;68:247-252

    Google Scholar 

  15. Cho WK, Stern S, Biggers JD: Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J Exp Zool 1974;187:383-386

    Google Scholar 

  16. Lindner HR, Tsafriri A, Lieberman ME, et al.: Gonadotropin action on cultured Graafian follicles: Induction of maturation division of the mammalian oocyte and differentiation of the luteal cell. Recent Prog Horm Res 1974;30:79-138

    Google Scholar 

  17. Dekel N, Galiani D, Sherizly I: Dissociation between the inhibitory and the stimulatory action of cAMP on maturation of rat oocytes. Mol Cell Endocrinol 1988;56:115-121

    Google Scholar 

  18. Cross PC: The role of cumulus cells and serum in mouse oocyte maturation in vitro. J Reprod Fertil 1973;34:241-245

    Google Scholar 

  19. Gavin AC, Vassalli JD, Cavadore JC, Schorderet-Slatkine S: Okadaic acid and p13suc1 modulate the reinitiation of meiosis in mouse oocytes. Mol Reprod Dev 1992;33:287-296

    Google Scholar 

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Authors and Affiliations

  1. First Department of Obstetrics and Gynecology, Toho University School of Medicine, 6-11-1, Ohmori-nishi, Ohta Word, Tokyo, 143, Japan

    Hideyuki Nakano & Harumi Kubo

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  1. Hideyuki Nakano
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  2. Harumi Kubo
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Nakano, H., Kubo, H. Study of the In Vitro Maturation of Mouse Oocytes Induced by Microinjection of Maturation Promoting Factor (MPF). J Assist Reprod Genet 17, 67–73 (2000). https://doi.org/10.1023/A:1009458215882

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