Abstract
Purpose: To determine whether embryos resulting fromoocytes matured in vitro have a higher incidence of nuclearand/or genetic abnormalities compared to embryos resultingfrom oocytes matured in vivo.
Methods: Fluorescence in situ hybridization analysis forchromosomes X, Y, and 18 was used to compare the ratesof aneuploidy, mosaicism, and nuclear abnormalities inembryos derived from oocytes that were prophase I ataspiration (immature group) to that observed in embryos resultingfrom oocytes that were metaphase I or II at aspiration(mature group).
Results: Based on nuclear morphology, significantly moreembryos in the mature group (23percnt;) were classified as normalcompared to embryos in the immature group (3percnt;). Nodifference was found in the rate of aneuploidy or in the incidenceof mosaicism involving these chromosomes.
Conclusions: These findings suggest that few embryosderived from prophase I oocytes collected following ovarianstimulation are morphologically normal.
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Descisciolo, C., Wright, D.L., Mayer, J.F. et al. Human Embryos Derived from In Vitro and In Vivo Matured Oocytes: Analysis for Chromosomal Abnormalities and Nuclear Morphology. J Assist Reprod Genet 17, 284–292 (2000). https://doi.org/10.1023/A:1009414517546
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DOI: https://doi.org/10.1023/A:1009414517546