Abstract
Purpose: A prospective, controlled in vitro study was conducted to evaluate the effects of human fallopian tube epithelium on the motility, velocity, and binding of humanspermatozoa.
Methods: Eleven fallopian tubes from six women undergoinghysterectomy and semen samples from 14 male partnersof women undergoing in vitro fertilization were collected.Human spermatozoa were cultured with monolayer of humanfallopian tube epithelial cells. The motility and velocity wereanalyzed subsequently at 0, 2, 6, 24, and 48 hr of incubation.The sperm binding capacity was analyzed after 48 hr in thehemizona assay (HZA).
Results: The presence of the human fallopian tube epithelialcells did not have any beneficial effects on sperm motilityand velocity. On the other hand, significant promoting effectwas observed in the ability of the sperm to bind to thezona pellucida.
Conclusions: The interaction of human spermatozoa withfallopian tube epithelial cells significantly increases spermbinding in the HZA.
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Ziskind, G., Paltieli, Y., Eibschitz, I. et al. Andrology: The Effect of Human Fallopian Tube Epithelium on Human Sperm Velocity Motility and Binding. J Assist Reprod Genet 17, 147–150 (2000). https://doi.org/10.1023/A:1009414121489
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DOI: https://doi.org/10.1023/A:1009414121489